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首页> 外文期刊>The European Journal of Neuroscience >Effects of Emx2 inactivation on the gene expression profile of neural precursors.
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Effects of Emx2 inactivation on the gene expression profile of neural precursors.

机译：Emx2失活对神经前体基因表达谱的影响。

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Emx2 plays a crucial role in the development of the diencephalon and dorsal telencephalon. Thus, Emx2-null mutants have abnormal cortical lamination and a reduction in size of the caudal and medial areas of the prosencephalon. Emx2 is expressed in neural precursors of the subventricular zone in vivo and in cultured neurospheres in vitro where it controls the size of the transit-amplifying population, affecting proliferation and clonal efficiency of neural stem cells. To identify the cellular processes mastered by Emx2, and possibly the molecular mechanisms by which the gene exerts its action, we compared the expression profile of cultured neurospheres derived from wild-type and Emx2-null mouse embryos. The differential expression of several genes was also confirmed by semiquantitative RT-PCR, real-time PCR and cytofluorimetric analysis in different preparations of neurospheres, and by in situ hybridization. The gene expression profile suggested a role for Emx2 in regulating the differentiation and migration properties of neural precursor cells. This involvement was confirmed in vitro, where the altered clonogenicity and impaired migration of Emx2-null cells were partially corrected by transduction of the Emx2 gene. Taken together, our results indicate that Emx2 is indeed involved in the transition between resident early progenitors (perhaps stem cells) and more mature precursors capable of migrating out of the ventricular zone, becoming postmitotic and differentiating into the appropriate cell type, and help explain the alterations observed in the brains of knock-out mice.
机译：Emx2在中脑和背脑的发育中起着至关重要的作用。因此，Emx2 null突变体具有异常的皮质层合，并且前脑的尾部和内侧区域的大小减小。 Emx2在体内脑室下区域的神经前体中表达，在体外培养的神经球中表达，它控制着转运放大种群的大小，影响神经干细胞的增殖和克隆效率。为了确定由Emx2掌握的细胞过程，以及可能由该基因发挥作用的分子机制，我们比较了源自野生型和Emx2空小鼠胚胎的培养神经球的表达谱。还通过半定量RT-PCR，实时PCR和细胞荧光分析在不同神经球制剂中以及通过原位杂交证实了几种基因的差异表达。基因表达谱表明Emx2在调节神经前体细胞的分化和迁移特性中的作用。这种参与在体外得到证实，其中Emx2无效细胞的克隆形成性和迁移受损可通过Emx2基因的转导部分纠正。综上所述，我们的结果表明Emx2确实参与了驻留的早期祖细胞（也许是干细胞）与能够移出心室区，成为有丝分裂后并分化为合适细胞类型的更成熟前体之间的过渡，并有助于解释在基因敲除小鼠的大脑中观察到的变化。

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