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首页> 外文期刊>The European Journal of Neuroscience >The slow afterhyperpolarization modulates high pH-induced changes in the excitability of rat CA1 pyramidal neurons.
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The slow afterhyperpolarization modulates high pH-induced changes in the excitability of rat CA1 pyramidal neurons.

机译：缓慢的超极化后调节高pH诱导的大鼠CA1锥体神经元兴奋性的变化。

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摘要

Extra- and intracellular recordings from the CA1 region of rat hippocampal slices were employed to examine the role of the slow afterhyperpolarization (sAHP) in modulating the increases in neuronal excitability observed on increasing extracellular pH (pHo) from 7.4 to 7.7. In the majority of experiments, an antidromic conditioning stimulus applied in the presence of D(-)-2-amino-5-phosphonopentanoic acid (D-APV), 6-cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX) and bicuculline was employed to elicit a sAHP, and an antidromic test stimulus was applied during the sAHP. At pHo 7.4, a single conditioning stimulus elicited an action potential followed by a sAHP, which in turn inhibited the response to the test stimulus compared with the conditioning stimulus. Increasing the number of action potentials in the conditioning stimulus augmented the sAHP and further inhibited the test response, whereas isoproterenol inhibited the sAHP and prevented the relative inhibition of the test response. At pHo 7.7, a single conditioning stimulus elicited a burst of action potentials followed by a large sAHP, which in turn prevented the test stimulus from eliciting a burst of action potentials and, in extracellular recordings, further increased the inhibition of the test response. The latter effect did not solely reflect a high pHo-induced increase in the conditioning response (and, thus, the subsequent sAHP), but rather involved a more direct effect of high pHo to augment the sAHP. The results indicate that increasing pHo increases the excitability of CA1 neurons to an initial stimulus; however, a high pHo-dependent increase in the sAHP evoked by the initial stimulus limits the response to subsequent stimuli.
机译：使用大鼠海马切片CA1区的细胞外和细胞内记录来研究慢后超极化（sAHP）在调节将细胞外pH（pHo）从7.4增加到7.7时观察到的神经元兴奋性增加中的作用。在大多数实验中，在D（-）-2-氨基-5-膦基戊酸（D-APV），6-氰基-7-硝基喹喔啉-2,3-二酮二钠盐（ CNQX）和bicuculline被用来引发sAHP，并且在sAHP期间应用了抗皮肤测试刺激。在pHo 7.4时，单个条件刺激会引起动作电位，随后是sAHP，与条件刺激相比，后者会抑制对测试刺激的反应。调节刺激中动作电位的增加会增加sAHP并进一步抑制测试反应，而异丙肾上腺素会抑制sAHP并阻止测试反应的相对抑制。在pHo 7.7时，单个条件刺激会引发一连串的动作电位，然后发出较大的sAHP，这反过来又阻止了测试刺激物诱发一连串的动作电位，并且在细胞外记录中，进一步增加了对测试响应的抑制。后者的作用不仅反映了pHo诱导的调理反应的增加（以及随后的sAHP），而且还涉及到高pHo增强sAHP的更直接的作用。结果表明，增加pHo可以增加CA1神经元对初始刺激的兴奋性。然而，初始刺激引起的sAHP的pHo依赖性高升高限制了对后续刺激的反应。

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