...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Differential expression of PKC beta II in the rat organ of Corti.
【24h】

Differential expression of PKC beta II in the rat organ of Corti.

机译：PKC beta II在Corti大鼠器官中的差异表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate a possible involvement of protein kinase C (PKC) in cochlear efferent neurotransmission, we studied the expression of the calcium-dependent PKC beta II isoform in the rat organ of Corti at different postnatal ages using immunofluorescence and immunoelectron microscopy. We found evidence of PKC beta II as early as postnatal day (PND) 5 in efferent axons running in the inner spiral bundle and in Hensen cells. At PND 8, we also found PKC beta II in efferents targeting outer hair cells (OHCs), and a slight detection at the synaptic pole in the first row of the basal and middle cochlear turns. At PND 12, PKC beta II expression declined in the efferent fibres contacting OHCs, whereas expression was concentrated at the postsynaptic membrane, from the basal and middle turns. The adult-like pattern of PKC beta II distribution was observed at PND 20. Throughout the cochlea, we found PKC beta II expression in the Hensen cells, non-sensory cells involved in potassium re-cycling, and lateral efferentterminals of the inner spiral bundle. In addition, we observed expression in OHCs at the postsynaptic membrane facing the endings of the medial efferent system, with the exception of some OHCs located in the most apical region of the cochlea. These data therefore suggest an involvement of PKC beta II in both cochlear efferent neurotransmission and ion homeostasis. Among other functions, PKC beta II could play a role in the efferent control of OHC activity.
机译：为了研究蛋白激酶C（PKC）在耳蜗传出神经传递中的可能参与，我们使用免疫荧光和免疫电子显微镜研究了出生后不同年龄的Corti大鼠器官中钙依赖性PKC beta II亚型的表达。我们发现早在产后一天（PND）5内螺旋束和Hensen细胞中传出的轴突中就有PKC beta II的证据。在PND 8处，我们还在针对外毛细胞（OHC）的传出物中发现了PKC beta II，并且在基底和中间耳蜗转弯的第一行突触极检测到轻微的异常。在PND 12，PKC beta II表达在与OHCs接触的传出纤维中降低，而表达则集中在突触后膜的基部和中部。在PND 20处观察到了成年人样的PKC beta II分布模式。在整个耳蜗中，我们发现Hensen细胞，参与钾循环的非感觉细胞以及内部螺旋束的侧向传出末端均表达PKC beta II。 。此外，我们观察到在面向内侧传出系统末端的突触后膜的OHCs中表达，除了一些位于耳蜗最顶端区域的OHCs。因此，这些数据表明PKC beta II参与耳蜗传出的神经传递和离子稳态。在其他功能中，PKC beta II可能在OHC活性的传出控制中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号