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首页> 外文期刊>The European Journal of Neuroscience >The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter.
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The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter.

机译：果蝇中混合的GF-TTMn突触的化学成分使用乙酰胆碱作为其神经递质。

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摘要

The largest central synapse in adult Drosophila is a mixed electro-chemical synapse whose gap junctions require the product of the shaking-B (shak-B) gene. Shak-B(2) mutant flies lack gap junctions at this synapse, which is between the giant fibre (GF) and the tergotrochanteral motor neuron (TTMn), but it still exhibits a long latency response upon GF stimulation. We have targeted the expression of the light chain of tetanus toxin to the GF, to block chemical transmission, in shak-B(2) flies. The long latency response in the tergotrochanteral muscle (TTM) was abolished indicating that the chemical component of the synapse mediates this response. Attenuation of GAL4-mediated labelling by a cha-GAL80 transgene, reveals the GF to be cholinergic. We have used a temperature-sensitive allele of the choline acetyltransferase gene (cha(ts2)) to block cholinergic synapses in adult flies and this also abolished the long latency response in shak-B(2) flies. Taken together the data provide evidence that both components of this mixed synapse are functional and that the chemical neurotransmitter between the GF and the TTMn is acetylcholine. Our findings show that the two components of this synapse can be separated to allow further studies into the mechanisms by which mixed synapses are built and function.
机译：成年果蝇中最大的中央突触是混合的电化学突触，其间隙连接需要震动-B（shak-B）基因的产物。 Shak-B（2）突变体蝇在这种突触处缺少间隙连接，该突触在巨纤维（GF）与转子转子运动神经元（TTMn）之间，但在GF刺激下它仍表现出长时延响应。我们针对破伤风毒素轻链的表达针对GF，以阻止shak-B（2）苍蝇中的化学传递。取消了转子粗隆肌（TTM）中的长潜伏期响应，表明突触的化学成分介导了该响应。 cha-GAL80转基因对GAL4介导的标记的减弱显示GF是胆碱能的。我们已经使用了胆碱乙酰基转移酶基因（cha（ts2））的温度敏感等位基因来阻止成年果蝇的胆碱能突触，这也消除了shak-B（2）果蝇的长潜伏期反应。数据合在一起提供了证据，证明这种混合突触的两个组件都具有功能，并且GF和TTMn之间的化学神经递质是乙酰胆碱。我们的研究结果表明，可以分开此突触的两个组件，以允许进一步研究混合突触的构建和功能机理。

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