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首页> 外文期刊>The European Journal of Neuroscience >Pre- and postsynaptic excitation and inhibition at octopus optic lobe photoreceptor terminals; implications for the function of the 'presynaptic bags'.
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Pre- and postsynaptic excitation and inhibition at octopus optic lobe photoreceptor terminals; implications for the function of the 'presynaptic bags'.

机译：章鱼视叶光感受器末端的突触前和突触后兴奋和抑制;对“突触前袋”功能的影响。

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Synaptic transmission was examined in the plexiform zone of Octopus vulgaris optic lobes using field-potential recording from optic lobe slices. Stimulation of the optic nerve produced pre- and postsynaptic field potentials. Transmission was abolished in calcium-free seawater, L- glutamate or the AMPA/Kainate receptor blocker CNQX (EC(50), 40 microm), leaving an intact presynaptic field potential. ACh markedly reduced or blocked and d-tubocurarine augmented both pre- and postsynaptic field potentials, while alpha-bungarotoxin and atropine were without effect. Paired-pulse stimulation showed short-term depression of pre- and postsynaptic components with a half-time of recovery of approximately 500 ms. The depression was partially relieved in the presence of d-tubocurarine (half-time of recovery, 350 ms). No long-term changes in synaptic strength were induced by repetitive stimulation. A polyclonal antibody raised against a squid glutamate receptor produced positive staining in the third radial layer of the plexiform zone. No positive staining was observed in the other layers. Taking into account previous morphological data and our results, we propose that the excitatory terminations of the photoreceptors are in the innermost layer of the plexiform zone where the transmitter is likely to be glutamate and postsynaptic receptors are AMPA/kainate-like. Thus, the function of the terminal bags is to provide a location for a presynaptic cholinergic inhibitory shunt. The results imply that this arrangement provides a temporal filter for visual processing and enhances the perception of moving vs. stationary objects.
机译：使用视神经叶切片的场电势记录检查了章鱼视神经叶丛区域的突触传递。视神经的刺激产生突触前和突触后领域的潜力。在无钙海水，L-谷氨酸盐或AMPA / Kainate受体阻滞剂CNQX（EC（50），40微米）中消除了传播，留下了完整的突触前场电位。乙酰胆碱显着降低或受阻，d-微管尿素增加突触前和突触后的电场潜能，而α-邦加罗毒素和阿托品则无作用。配对脉冲刺激显示突触前和突触后成分的短期抑制，恢复的半衰期约为500毫秒。在存在d-微管尿素的情况下，抑郁症得到部分缓解（恢复时间为350毫秒）。重复刺激未引起突触强度的长期变化。产生针对鱿鱼谷氨酸受体的多克隆抗体，在丛状区的第三个径向层产生阳性染色。在其他层中未观察到阳性染色。考虑到以前的形态学数据和我们的结果，我们建议光感受器的兴奋性终止位于丛状区的最内层，在该层中，递质可能是谷氨酸，而突触后受体是AMPA /海藻酸盐样。因此，终端袋的功能是为突触前胆碱能抑制分流提供位置。结果表明，这种安排为视觉处理提供了时间过滤器，并增强了移动物体与静止物体的感知。

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