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首页> 外文期刊>The European Journal of Neuroscience >Glutamatergic neurons are present in the rat ventral tegmental area.
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Glutamatergic neurons are present in the rat ventral tegmental area.

机译：谷氨酸能神经元存在于大鼠腹侧被盖区。

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摘要

The ventral tegmental area (VTA) is thought to play an important role in reward function. Two populations of neurons, containing either dopamine (DA) or gamma-amino butyric acid (GABA), have been extensively characterized in this area. However, recent electrophysiological studies are consistent with the notion that neurons that utilize neurotransmitters other than DA or GABA are likely to be present in the VTA. Given the pronounced phenotypic diversity of neurons in this region, we have proposed that additional cell types, such as those that express the neurotransmitter glutamate may also be present in this area. Thus, by using in situ hybridization histochemistry we investigated whether transcripts encoded by genes for the two vesicular glutamate transporters, VGluT1 or VGluT2, were expressed in the VTA. We found that VGluT2 mRNA but not VGluT1 mRNA is expressed in the VTA. Neurons expressing VGluT2 mRNA were differentially distributed throughout the rostro-caudal and medio-lateral aspects of the VTA,with the highest concentration detected in rostro-medial areas. Phenotypic characterization with double in situ hybridization of these neurons indicated that they rarely co-expressed mRNAs for tyrosine hydroxylase (TH, marker for DAergic neurons) or glutamic acid decarboxylase (GAD, marker for GABAergic neurons). Based on the results described here, we concluded that the VTA contains glutamatergic neurons that in their vast majority are clearly non-DAergic and non-GABAergic.
机译：腹侧被盖区（VTA）被认为在奖励功能中起重要作用。该区域已广泛表征了包含多巴胺（DA）或γ-氨基丁酸（GABA）的两个神经元群体。但是，最近的电生理研究与VTA中可能存在利用DA或GABA以外的神经递质的神经元的观点一致。考虑到该区域神经元明显的表型多样性，我们建议在该区域也可能存在其他细胞类型，例如表达神经递质谷氨酸的细胞类型。因此，通过使用原位杂交组织化学，我们研究了两种水泡谷氨酸转运蛋白VGluT1或VGluT2的基因编码的转录物是否在VTA中表达。我们发现VGluT2 mRNA而不是VGluT1 mRNA在VTA中表达。表达VGluT2 mRNA的神经元在VTA的整个尾状和中外侧分布有差异，其中最高浓度出现在rostro-medial区域。这些神经元的双原位杂交表型特征表明，它们很少共表达酪氨酸羟化酶（TH，DAergic神经元的标记）或谷氨酸脱羧酶（GAD，GABA能神经元的标记）的mRNA。根据此处描述的结果，我们得出的结论是，VTA包含谷氨酸能神经元，其中绝大多数明显是非DAergic和非GABAergic。

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