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首页> 外文期刊>The European physical journal, E. Soft matter >Diffusion in supported lipid bilayers: Influence of substrate and preparation technique on the internal dynamics
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Diffusion in supported lipid bilayers: Influence of substrate and preparation technique on the internal dynamics

机译:在支持的脂质双层中的扩散:底物和制备技术对内部动力学的影响

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摘要

The diffusion law of DMPC and DPPC in Supported Lipid Bilayers (SLB), on different substrates, has been investigated in details by Fluorescence Recovery After Patterned Photobleaching (FRAPP). Over micrometer length scales, we demonstrate the validity of a purely Brownian diffusive law both in the gel and the fluid phases of the lipids. Measuring the diffusion coefficient as a function of temperature, we characterize the gel-to-liquid phase transition of DMPC and DPPC. It is shown that, depending on the type of substrate and the method used for bilayer preparation, completely different behaviours can be observed. On glass substrates, using the Langmuir-Blodgett deposition technique, both leaflets of the bilayer have the same dynamics. On mica, the dynamics of the proximal leaflet is slower than the dynamics of the distal leaflet, although the transition temperature is the same for both layers. Preparing bilayers from vesicle fusion in same conditions leads to more random behaviours and shifted transition temperatures.
机译:DMPC和DPPC在不同基质上的脂质双分子膜(SLB)中的扩散规律已通过图案化光漂白后的荧光恢复(FRAPP)进行了详细研究。在微米长度尺度上,我们证明了纯的布朗扩散定律在脂质的凝胶和流体相中都是有效的。通过测量扩散系数随温度的变化,我们可以表征DMPC和DPPC的凝胶到液相的转变。结果表明,取决于基底的类型和用于双层制备的方法,可以观察到完全不同的行为。在玻璃基板上,使用Langmuir-Blodgett沉积技术,双层的两个小叶都具有相同的动力学。在云母上,尽管两层的过渡温度相同,但近端小叶的动力学要比远端小叶的动力学慢。在相同条件下从囊泡融合制备双层膜会导致更多的随机行为和转变温度的变化。

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