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首页> 外文期刊>The FEBS journal >Genome-wide expressional and functional analysis ofcalcium transport elements during abiotic stress anddevelopment in rice
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Genome-wide expressional and functional analysis ofcalcium transport elements during abiotic stress anddevelopment in rice

机译：水稻非生物胁迫和发育过程中钙转运元素的全基因组表达和功能分析

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Ca~(2+) homeostasis is required to maintain a delicate balance of cytosolicCa~(2+) during normal and adverse growth conditions. Various Ca~(2+) transportersactively participate to maintain this delicate balance especially duringabiotic stresses and developmental events in plants. In this study, wepresent a genome-wide account, detailing expression profiles, subcellularlocalization and functional analysis of rice Ca~(2+) transport elements.Exhaustive in silico data mining and analysis resulted in the identificationof 81 Ca~(2+) transport element genes, which belong to various groups suchas Ca~(2+)-ATPases (pumps), exchangers, channels, glutamate receptor homologsand annexins. Phylogenetic analysis revealed that different Ca~(2+)transporters are evolutionarily conserved across different plant species.Comprehensive expression analysis by gene chip microarray and quantitativeRT-PCR revealed that a substantial proportion of Ca~(2+) transportergenes were expressed differentially under abiotic stresses (salt, cold anddrought) and reproductive developmental stages (panicle and seed) in rice.These findings suggest a possible role of rice Ca~(2+) transporters in abioticstress and development triggered signaling pathways. Subcellular localizationof Ca~(2+) transporters from different groups in Nicotiana benthamianarevealed their variable localization to different compartments, which couldbe their possible sites of action. Complementation of Ca~(2+) transport activityof K616 yeast mutant by Ca~(2+)-ATPase OsACA7 and involvement insalt tolerance verified its functional behavior. This study will encouragedetailed characterization of potential candidate Ca~(2+) transporters for theirfunctional role in planta.
机译：在正常和不利的生长条件下，需要Ca〜（2+）动态平衡来维持细胞溶质Ca〜（2+）的微妙平衡。各种Ca〜（2+）转运蛋白积极参与维持这种微妙的平衡，尤其是在植物的非生物胁迫和发育过程中。在这项研究中，我们提出了一个全基因组的账户，详细描述了水稻Ca〜（2+）转运元件的表达谱，亚细胞定位和功能分析。详尽的计算机数据挖掘和分析结果确定了81 Ca〜（2+）转运元件。这些基因属于Ca〜（2 +）-ATPases（泵），交换子，通道，谷氨酸受体同源物和膜联蛋白等不同组。系统发育分析表明，不同植物中Ca〜（2+）转运蛋白的进化保守性。基因芯片和定量RT-PCR的综合表达分析表明，非生物胁迫下Ca〜（2+）转运能的表达差异很大。 （盐，冷和干旱）和水稻的生殖发育阶段（穗和种子）。这些发现表明，水稻Ca〜（2+）转运蛋白在非生物胁迫和发育触发信号通路中的可能作用。本底烟草中不同群体的Ca〜（2+）转运蛋白的亚细胞定位揭示了其可变的定位于不同的区室，这可能是它们可能的作用部位。 Ca〜（2 +）-ATPase OsACA7对K616酵母突变体的Ca〜（2+）转运活性的补充和耐盐性证明了其功能行为。这项研究将鼓励对潜在候选Ca〜（2+）转运蛋白在植物中的功能性角色进行详细的表征。

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