...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Insulin-dependent phosphorylation of DPP IV in liver
【24h】

Insulin-dependent phosphorylation of DPP IV in liver

机译：肝脏中DPP IV的胰岛素依赖性磷酸化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dipeptidyl peptidase IV (DPP IV, CD26, EC 3.4.14.5) serves as a model aimed at elucidating protein sorting signals. We identify here, by MS, several tyrosine-phosphorylated proteins in a rat liver Golgi/endosome (G/E) fraction including DPP IV. We show that a pool of DPP IV is tyrosine-phosphorylated. Maximal phosphorylation was observed after 2 min following intravenous insulin injection. DPP IV coimmunoprecipitated with the cellular tyrosine kinase Src (c-Src) with maximal association also observed after 2 min following insulin injection. DPP IV was found phosphorylated after incubation of nonsolubilized G/E membranes with [gamma-P-32]ATP. The c-Src inhibitor PP2 inhibited DPP IV phosphorylation. Oriented proteolysis experiments indicate that a large pool of c-Src is protected in G/E fractions. Following injection of the protein-tyrosine phosphatase inhibitor bpV(phen), DPP IV levels markedly decreased by 40% both in plasma membrane and G/E fractions. In the fraction designated Lh, DPP IV levels decreased by 50% 15 min following insulin injection. Therefore, a pool of DPP IV is tyrosine-phosphorylated in an insulin-dependent manner. The results suggest the presence of a yet to be characterized signalling mechanism whereby DPP IV has access to c-Src-containing signalling platforms.
机译：二肽基肽酶IV（DPP IV，CD26，EC 3.4.14.5）用作旨在阐明蛋白质分选信号的模型。我们在这里通过MS在大鼠肝高尔基体/内体（G / E）组分（包括DPP IV）中鉴定了几种酪氨酸磷酸化蛋白。我们表明，DPP IV池是酪氨酸磷酸化的。静脉注射胰岛素后2分钟观察到最大磷酸化。注射胰岛素后2分钟，也观察到与细胞酪氨酸激酶Src（c-Src）具有最大缔合性的DPP IV免疫共沉淀。在将未溶解的G / E膜与[γ-P-32] ATP孵育后，发现DPP IV磷酸化。 c-Src抑制剂PP2抑制DPP IV磷酸化。定向蛋白水解实验表明，大量的c-Src在G / E馏分中受到保护。注射蛋白酪氨酸磷酸酶抑制剂bpV（phen）后，质膜和G / E部分的DPP IV水平均明显降低40％。在注射Lh的馏分中，胰岛素注射后15分钟DPP IV水平降低了50％。因此，DPP IV库以胰岛素依赖的方式被酪氨酸磷酸化。结果表明存在尚待表征的信号传导机制，DPP IV可以访问含c-Src的信号传导平台。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号