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首页> 外文期刊>The FEBS journal >Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells
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Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells

机译：HeLa细胞从热休克恢复过程中，poly（A）结合蛋白的表达上调

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Induction of heat shock proteins (HSPs) helps cells to survive severe hyperthermal stress and removes toxic unfolded proteins. At the same time, the cap-dependent translation of global cellular mRNA is inhibited, due to the loss of function of eukaryotic initiation factor (eIF)4F complex. It has been previously reported that, following heat shock, HSP27 binds to the insoluble granules of eIF4G and impedes its association with cytoplasmic poly(A)-binding protein (PABP) 1 and eIF4E. In the studies reported here, in addition to heat shock, we have included results of our investigation on the association between eIF4G, PABP1 and HSP27 during recovery from heat shock, when cap-dependent mRNA translation resumes. We showed here that in the heat-shocked cells, the PABP1-eIF4G complex dissociated, and both polypeptides translocated with the HSP27 to the nucleus. During recovery after heat shock, PABP1 and eIF4G were redistributed into the cytoplasm and colocalized with each other. In addition, PABP1 expression was upregulated and its translation efficiency was increased during the recovery period, possibly to meet additional demands on the translation machinery. HSP27 remained associated with the eIF4G-PABP1 complex during recovery from heat shock. Therefore, our results raise the possibility that the association of HSP27 with eIF4G may not be sufficient to suppress cap-dependent translation during heat shock. In addition, we provide evidence that the terminal oligopyrimidine cis-element of PABP1 mRNA is responsible for the preferential increase of PABP1 mRNA translation in cells undergoing recovery from heat shock.
机译：热休克蛋白（HSP）的诱导可帮助细胞在严重的高温胁迫下存活并去除有毒的未折叠蛋白。同时，由于真核起始因子（eIF）4F复合体功能的丧失，总细胞mRNA的帽依赖性翻译受到抑制。以前已经报道过，在热激之后，HSP27结合到eIF4G的不溶颗粒上，并阻止其与细胞质聚（A）结合蛋白（PABP）1和eIF4E结合。在这里报道的研究中，除了热休克外，我们还包括了当热休克恢复帽依赖性mRNA翻译时，eIF4G，PABP1和HSP27之间的联系的调查结果。我们在这里显示，在热激细胞中，PABP1-eIF4G复合物解离，并且两个多肽都与HSP27一起转运到细胞核。在热休克后的恢复过程中，PABP1和eIF4G重新分布到细胞质中并彼此共定位。此外，在恢复期PABP1的表达上调并提高了翻译效率，这可能满足了对翻译机制的额外要求。从热休克恢复过程中，HSP27仍与eIF4G-PABP1复合物相关。因此，我们的结果提出了HSP27与eIF4G的关联可能不足以抑制热激过程中依赖于帽的翻译的可能性。此外，我们提供的证据表明，PABP1 mRNA的末端寡嘧啶顺式元件可导致热休克恢复的细胞中PABP1 mRNA的翻译优先增加。

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