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首页> 外文期刊>The FEBS journal >ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults.
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ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults.

机译：ArHsp22是在滞育期的Artemia胚胎中产生的一种发育受调控的小热休克蛋白，在成年人中可诱发压力。

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摘要

Diapause embryos of the crustacean Artemia franciscana exhibit extreme stress tolerance, a property thought to involve molecular chaperones known as small heat shock proteins. To further explore this idea, the structure, function and synthesis of ArHsp22, an Artemia small heat shock protein, were characterized. ArHsp22 contains amino-terminal WXDPF motifs, an alpha-crystallin domain with a highly conserved arginine, and a carboxy-terminal I/VXI/V motif, all typical of small heat shock proteins. ArHsp22 formed large oligomers and exhibited molecular chaperone activity in vitro, protecting citrate synthase and insulin from denaturation. Quantitative PCR and immunoprobing of western blots revealed that ArHsp22 synthesis is restricted to diapause-destined Artemia embryos and that the protein is degraded during post-diapause development. ArHsp22 was observed in cyst nuclei, a location shared by p26 but not ArHsp21, which are two other diapause-specific Artemia small heat shock proteins. ArHsp22 production was enhanced by thermal stress, but only in adults, thus representing the first crustacean small heat shock protein whose synthesis is known to be both developmentally regulated and stress inducible. The results demonstrate that expression of the gene for ArHsp22 is modulated by multiple cues that vary with life history stage. Such findings are of importance in understanding diapause maintenance in Artemia embryos and the survival of adult animals experiencing environmental insult.
机译：甲壳类动物南半球的滞育性胚胎表现出极高的压力耐受性，这种特性被认为与分子伴侣有关，称为小热激蛋白。为了进一步探索这一思想，对卤虫小热激蛋白ArHsp22的结构，功能和合成进行了表征。 ArHsp22包含氨基末端的WXDPF基序，具有高度保守精氨酸的α-晶状蛋白结构域和羧基末端的I / VXI / V基序，所有这些都是小型热激蛋白的典型特征。 ArHsp22形成大的低聚物，并在体外表现出分子伴侣活性，从而保护柠檬酸合酶和胰岛素免于变性。定量PCR和Western印迹的免疫探测显示，ArHsp22合成仅限于以滞育为目标的Artemia胚胎，并且该蛋白质在滞后发育过程中会降解。在囊核中观察到了ArHsp22，这是p26共有的位置，但不是ArHsp21，这是另外两个滞育特异性的Artemia小热激蛋白。 ArHsp22的产生通过热胁迫而得以增强，但仅在成年人中，因此代表了第一个甲壳类小热激蛋白，其合成已知受发育调节且可诱导应激。结果表明，ArHsp22基因的表达受到多种提示的调节，这些提示随生命历史阶段而变化。这些发现对于理解卤虫胚胎中滞育的维持以及遭受环境侵害的成年动物的存活至关重要。

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