...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bulletin of the Chemical Society of Japan >Interaction of Dicationic Bis(imidazoliumyl)porphyrinatometals with DNA
【24h】

Interaction of Dicationic Bis(imidazoliumyl)porphyrinatometals with DNA

机译：阳离子双（咪唑烷基）卟啉金属与DNA的相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A pair of dicationic metalloporphyrins with only two meso-substituents,i.e.copper(II) and zinc(II) complexes of 5,15-bis(1,3-dimethylimidazolium-2-yl)porphyrin (H_2-1),have been synthesized by insertion reaction of the metal ions.Interactions of [5,15-bis(1,3-dimethylimidazolium-2-yl)porphyrinato]copper(II) (Cu-1) and [5,15-bis(1,3-dimethylimidazolium-2-yl)porphyrinato]zine(II) (Zn-1) with calf thymus DNA (CT-DNA) and double-helical synthetic polynucleotides ([poly(dA-dT)]_2 and [poly(dG-dC)]_2) have been studied by melting temperature,viscometric,visible absorption,CD and MCD spectroscopic measurements.The copper(II) complex,Cu-1,intercalates into the base pairs of DNA such as CT-DNA,[poly(dA-dT)]_2 and [poly(dG-dT)]_2 and [poly(dG-dC)]_2 to stabilize the DNA duplex due to its high affinity for them.In contrast,the zinc(II) complex,Zn-1,binds edge-on to CT-DNA,[poly(dA-dT)]_2 and [poly(dG-dC)]_2 as an outside binder.The binding constant of Cu-1 to DNA is in the order of 10~6 M~(-1) in a phosphate buffer (pH 6.8) at 25 deg C,while that of Zn-1ranges from 10~4 to 10~5 M~(-1) under the same experimental conditions.The binding of Cu-1 and Zn-1 to DNA is entropically driven,although that of H_2-1 is enrthalpically driven.As a result,it has been revealed that the kind of central metal ions in dicationic metalloporphyrins influences the binding properties of the dicationic porphyrin to DNA.
机译：合成了一对仅含5,15-双（1,3-二甲基咪唑鎓-2-基）卟啉（H_2-1）的铜（II）和锌（II）配合物的金属卟啉对[5,15-双（1,3-二甲基咪唑鎓-2-基）卟啉对]铜（II）（Cu-1）和[5,15-双（1,3-）的相互作用牛小胸腺DNA（CT-DNA）和双螺旋合成多核苷酸（[poly（dA-dT）] _ 2和[poly（dG-dC） ] _2）已通过熔融温度，粘度测定，可见吸收，CD和MCD光谱测量进行了研究。铜（II）配合物Cu-1嵌入到DNA的碱基对中，例如CT-DNA，[poly（dA- dT）] _ 2和[poly（dG-dT）] _ 2和[poly（dG-dC）] _ 2对DNA双链体具有很高的亲和力，从而使其稳定。 ，边缘结合到CT-DNA，[poly（dA-dT）] _ 2和[poly（dG-dC）] _ 2作为外部结合剂。Cu-1与DNA的结合常数约为10〜 6 M〜（-1）在在相同的实验条件下，25°C的磷酸盐缓冲液（pH 6.8），Zn-1的缓冲液在10〜4到10〜5 M〜（-1）范围内。Cu-1和Zn-1与DNA的结合结果表明，金属卟啉中的中心金属离子的种类会影响金属卟啉与DNA的结合特性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号