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首页> 外文期刊>The FEBS journal >Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with protein kinase C2, a splice variant of ubiquitin ligase RBCC protein interacting with protein kinase C1
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Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with protein kinase C2, a splice variant of ubiquitin ligase RBCC protein interacting with protein kinase C1

机译：通过过表达与蛋白激酶C2相互作用的RBCC蛋白来积累多泛素化蛋白，泛素连接酶RBCC蛋白与蛋白激酶C1相互作用的剪接变体

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The nuclear-cytoplasmic shuttling protein RBCC protein interacting with protein kinase C1 (RBCK1) possesses transcriptional and ubiquitin ligase activities. We have recently reported that RBCC protein interacting with protein kinase C2 (RBCK2), a RING-in-between-RING fingers domain-lacking splice variant of RBCK1, lacks transcriptional activity, but rather represses the RBCK1-mediated transcriptional activity as a cytoplasmic tethering protein for RBCK1. In this study, we have found that RBCK2 overexpressed in human embryonic kidney 293 cells interacts with the polyubiquitin chain and the polyubiquitin-interacting subunit S5a, and significantly increases the intracellular amount of polyubiquitinated proteins. These results strongly suggested that RBCK2 functions as an adaptor protein for the polyubiquitinated protein and the S5a subunit in 26S proteasome through its novel zinc finger motif and ubiquitin-like domain, respectively, presumably delivering the polyubiquitinated proteins to the entrance of the 26S proteasome catalytic domain for degradation. Structured digital abstract MINT-7261426: S5a (uniprotkb: P55036) binds ( MI:0407) to Rbck1 (uniprotkb: Q62921) by pull down ( MI:0096) MINT-7261435: S5a (uniprotkb: P55036) physically interacts ( MI:0915) with Rbck1 (uniprotkb: Q62921) by anti bait coimmunoprecipitation ( MI:0006) MINT-7261448: S5a (uniprotkb: P55036) physically interacts ( MI:0915) with Rbck1 (uniprotkb: Q62921) by anti tag coimmunoprecipitation ( MI:0007) MINT-7261462: Rbck1 (uniprotkb: Q62921) physically interacts ( MI:0915) with Ubiquitin (uniprotkb: P62988) by anti tag coimmunoprecipitation ( MI:0007) MINT-7261503: S5a (uniprotkb: P55036) binds ( MI:0407) to Ubiquitin (uniprotkb: P62988) by pull down ( MI:0096) MINT-7261477: Rbck1 (uniprotkb: Q62921) binds ( MI:0407) to Ubiquitin (uniprotkb: P62988) by pull down ( MI:0096)
机译：与蛋白激酶C1（RBCK1）相互作用的核质穿梭蛋白RBCC蛋白具有转录和泛素连接酶活性。最近，我们报道了RBCC蛋白与蛋白激酶C2（RBCK2）相互作用，这是指RING环之间缺乏RING的CKCK1剪接变体，它缺乏转录活性，而是抑制了RBCK1介导的转录活性，作为胞质束缚RBCK1蛋白。在这项研究中，我们发现在人类胚胎肾293细胞中过表达的RBCK2与多聚泛素链和与多聚泛素相互作用的亚基S5a相互作用，并显着增加了多聚泛素化蛋白的细胞内数量。这些结果强烈表明，RBCK2通过其新的锌指基序和泛素样结构域分别充当26S蛋白酶体中多泛素化蛋白和S5a亚基的衔接蛋白，大概是将多泛素化蛋白递送至26S蛋白酶体催化结构域的入口。降解。结构化数字摘要MINT-7261426：S5a（uniprotkb：P55036）通过下拉（MI：0096）绑定（MI：0407）到Rbck1（uniprotkb：Q62921）MINT-7261435：S5a（uniprotkb：P55036）进行物理交互（MI：0915 ）与Rbck1（uniprotkb：Q62921）通过抗诱饵共免疫沉淀（MI：0006）MINT-7261448：S5a（uniprotkb：P55036）通过抗标签共免疫沉淀（MI：0007）与Rbck1（uniprotkb：Q62921）物理相互作用（MI：0915） MINT-7261462：Rbck1（uniprotkb：Q62921）通过抗标签共免疫沉淀（MI：0007）与Ubiquitin（uniprotkb：P62988）物理相互作用（MI：0915）MINT-7261503：S5a（uniprotkb：P55036）结合（MI：0407）与通过下拉（MI：0096）产生的泛素（uniprotkb：P62988）MINT-7261477：通过下拉（MI：0096）将Rbck1（uniprotkb：Q62921）绑定（MI：0407）与泛素（uniprotkb：P62988）

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