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首页> 外文期刊>Biomaterials Science >Cellulose-based polyelectrolyte complex nanoparticles for DNA vaccine delivery
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Cellulose-based polyelectrolyte complex nanoparticles for DNA vaccine delivery

机译：用于DNA疫苗递送的基于纤维素的聚电解质复合物纳米颗粒

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Cellulose-based nanoparticles were prepared from oppositely charged carboxymethyl cellulose (CMC) and quaternized cellulose (QC) in an aqueous medium. The DNA complexing capacity of CMC-QC nanoparticles and the transfection efficiency of the DNA-loaded nanoparticles in COS-7 cells were first investigated using pEGFP-N1-a plasmid DNA encoding the enhanced green fluorescent protein (EGFP)-as a model, and then with a candidate DNA vaccine, pMASIA-tPAs-tE2.2, that has been developed against infection with bovine viral diarrhea virus (BVDV). The results revealed that CMC-QC nanoparticles could bind DNA efficiently, and the optimized DNA-loaded nanoparticles mediated very effective transfection in COS-7 cells, which was comparable to that achieved with Lipofectamine 2000. The novel CMC-QC nanoparticles show promise as a delivery system for DNA vaccines.
机译：基于纤维素的纳米颗粒是由水性介质中带相反电荷的羧甲基纤维素（CMC）和季铵化纤维素（QC）制备的。首先使用pEGFP-N1-a编码增强型绿色荧光蛋白（EGFP）的质粒DNA作为模型研究CMC-QC纳米颗粒的DNA络合能力和COS-7细胞中DNA负载的纳米颗粒的转染效率，并然后使用候选的DNA疫苗pMASIA-tPAs-tE2.2，该疫苗已针对牛病毒性腹泻病毒（BVDV）的感染而开发。结果表明，CMC-QC纳米粒子可以有效地结合DNA，并且优化的DNA负载纳米粒子可在COS-7细胞中介导非常有效的转染，与Lipofectamine 2000相当。 DNA疫苗递送系统。

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