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首页> 外文期刊>Vaccine >Pulmonary delivery of chitosan-DNA nanoparticles enhances the immunogenicity of a DNA vaccine encoding HLA-A*0201-restricted T-cell epitopes of Mycobacterium tuberculosis
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Pulmonary delivery of chitosan-DNA nanoparticles enhances the immunogenicity of a DNA vaccine encoding HLA-A*0201-restricted T-cell epitopes of Mycobacterium tuberculosis

机译：肺部递送壳聚糖-DNA纳米颗粒增强了编码结核分枝杆菌HLA-A * 0201限制性T细胞表位的DNA疫苗的免疫原性

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摘要

In this study, we used an HLA-A2 transgenic mouse model to investigate the effects of pulmonary delivery of a new DNA plasmid encoding eight HLA-A*0201-restricted T-cell epitopes from Mycobacterium tuberculosis formulated in chitosan nanoparticles. It was shown that the chitosan-DNA formulation was able to induce the maturation of dendritic cells (DCs) while chitosan solution alone could not, indicating the DNA was released from the particles and able to stimulate DCs. Pulmonary administration of the DNA plasmid incorporated in chitosan nanoparticles was shown to induce increased levels of IFN-gamma secretion compared to pulmonary delivery of plasmid in solution or the more frequently used intramuscular immunization route. These results indicate that pulmonary delivery of DNA vaccines against tuberculosis may provide an advantageous delivery route compared to intramuscular immunization, and that increased immunogenicity can be achieved by delivery of this DNA encapsulated in chitosan nanoparticles
机译：在这项研究中，我们使用了HLA-A2转基因小鼠模型，研究了从壳聚糖纳米粒子中配制的结核分枝杆菌中编码8个HLA-A * 0201限制性T细胞表位的新DNA质粒的肺部递送的影响。结果表明，壳聚糖-DNA制剂能够诱导树突状细胞（DCs）的成熟，而单独的壳聚糖溶液则不能，表明DNA从颗粒中释放出来并能够刺激DCs。与在溶液中肺部递送质粒或较常使用的肌内免疫途径相比，肺部掺入脱乙酰壳多糖纳米颗粒中的DNA质粒显示诱导增加的IFN-γ分泌水平。这些结果表明，与肌肉内免疫相比，肺结核抗结核疫苗的递送可能提供了一种有利的递送途径，并且通过递送封装在壳聚糖纳米颗粒中的这种DNA可以提高免疫原性。

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