Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice.

Bivas-Benita M.; Lin Young; Bal S. M.; Meijgaarden K. E. van; Franken K. L. M. C.; Friggen A. H.; Junginger H. E.; Borchard G.; Klein M. R.; Ottenhoff T. H. M.

首页> 外文期刊>Vaccine >Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice.

【24h】

Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice.

机译：与PLGA-PEI纳米粒子相关的编码结核分枝杆菌潜伏期抗原Rv1733c的DNA的肺部递送增强了小鼠DNA初免/蛋白质加强免疫接种方案中的T细胞反应。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

During persistent infection and hypoxic-stress, Mycobacterium tuberculosis (Mtb) expresses a series of Mtb latency antigens. The aim of this study was to evaluate the immunogenicity of a DNA vaccine encoding the Mtb latency antigen Rv1733c and to explore the effect of pulmonary delivery and co-formulation with poly (D,L-lactide-co-glycolide) (PLGA)-polyethyleneimine (PEI) nanoparticles (np) on host immunity. Characterization studies indicated that PLGA-PEI np kept their nanometer size after concentration and were positively charged. The np were able to mature human dendritic cells and stimulated them to secrete IL-12 and TNF- alpha comparable to levels observed after lipopolysaccharide (LPS) stimulation. Mtb latency antigen Rv1733c DNA prime combined with Rv1733c protein boost enhanced T cell proliferation and IFN- gamma secretion in mice in response to Rv1733c and Mtb hypoxic lysate. Rv1733c DNA adsorbed to PLGA-PEI np and applied to the lungs increased T cell proliferation and IFN- gamma production more potently compared to the same vaccinations given intramuscularly. The strongest immunogenicity was obtained by pulmonary priming with np-adsorbed Rv1733c DNA followed by boosting with Rv1733c protein. These results confirm that PLGA-PEI np are an efficient DNA vaccine delivery system to enhance T cell responses through pulmonary delivery in a DNA prime/protein boost vaccine regimen.

机译：在持续感染和低氧应激期间，结核分枝杆菌（ Mtb ）表达一系列 Mtb 潜伏期抗原。这项研究的目的是评估编码 Mtb 潜伏期抗原Rv1733c的DNA疫苗的免疫原性，并探讨肺部递送和与聚（D，L-丙交酯-乙交酯（PLGA）-聚乙烯亚胺（PEI）纳米颗粒（np）对宿主的免疫力。表征研究表明，PLGA-PEI np浓缩后保持其纳米尺寸，并带正电。 np能够使人树突细胞成熟并刺激它们分泌与脂多糖（LPS）刺激后观察到的水平相当的IL-12和TNF-α。 Mtb潜伏期抗原Rv1733c DNA初免与Rv1733c蛋白结合可增强小鼠对Tv1733c和 Mtb 低氧裂解液的T细胞增殖和IFN-γ分泌。与肌肉内注射相同的疫苗相比，Rv1733c DNA吸附到PLGA-PEI np并应用于肺部后，可更有效地提高T细胞增殖和IFN-γ的产生。通过用np吸附的Rv1733c DNA进行肺启动，然后用Rv1733c蛋白加强免疫力，可获得最强的免疫原性。这些结果证实PLGA-PEI np是一种有效的DNA疫苗递送系统，可通过DNA初免/蛋白质加强疫苗方案中的肺部递送来增强T细胞反应。 展开▼

著录项

来源
《Vaccine》 |2009年第30期|共8页

作者
Bivas-Benita M.; Lin Young; Bal S. M.; Meijgaarden K. E. van; Franken K. L. M. C.; Friggen A. H.; Junginger H. E.; Borchard G.; Klein M. R.; Ottenhoff T. H. M.;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种 eng

中图分类预防医学、卫生学;

关键词
bacterial antigens; cytokines; disease models; DNA vaccines; immune response; immunity; immunization; in vitro; interferon; interleukin 12; laboratory animals; lungs; lymphocyte transformation; polymers; T lymphocytes; tuberculosis; tumour necrosis factor; vaccination; mice; Mycobacterium tuberculosis;

机译：细菌抗原;细胞因子;疾病模型;DNA疫苗;免疫反应;免疫;免疫;体外;干扰素;白介素12;实验室动物;肺;淋巴细胞转化;聚合物;T淋巴细胞;结核病;肿瘤坏死因子;疫苗接种;小鼠;分枝杆菌结核;

相似文献

外文文献

中文文献

专利

1. Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice. [J] . Bivas-Benita M., Lin Young, Bal S. M., Vaccine . 2009,第30期

机译：与PLGA-PEI纳米粒子相关的编码结核分枝杆菌潜伏期抗原Rv1733c的DNA的肺部递送增强了小鼠DNA初免/蛋白质加强免疫接种方案中的T细胞反应。

2. Enhanced protective efficacy against Mycobacterium tuberculosis afforded by BCG prime-DNA boost regimen in an early challenge mouse model is associated with increased splenic interleukin-2-producing CD4 T-cell frequency post-vaccination [J] . Kang Han, Yuan Qin, Ma Hui, Immunology: An Official Journal of the British Society for Immunology . 2014,第4期

机译：在早期攻击小鼠模型中，BCG初免-DNA增强方案提供的针对结核分枝杆菌的增强保护作用与疫苗接种后脾脏产生白介素2产生的CD4 T细胞频率增加有关

3. Pulmonary delivery of chitosan-DNA nanoparticles enhances the immunogenicity of a DNA vaccine encoding HLA-A*0201-restricted T-cell epitopes of Mycobacterium tuberculosis [J] . Bivas-Benita M, van Meijgaarden KE, Franken KLMC, Vaccine . 2004,第13a14期

机译：肺部递送壳聚糖-DNA纳米颗粒增强了编码结核分枝杆菌HLA-A * 0201限制性T细胞表位的DNA疫苗的免疫原性

4. POLY(D,L-LACTIC ACID) ENHANCES IMMUNE RESPONSES TO LIPOSOMAL DNA ENCODED ANTIGEN: MULTICOMPONENT PLASMID DNA DELIVERY SYSTEMS [C] . V. Bramwell, J. Eyles, S. Somavarapu, International symposium on controlled release of bioactive materials;Consumer diversified products conference . 2001

机译：聚（D，L-乳酸）增强了对脂质体DNA编码抗原的免疫反应：多组分质粒DNA递送系统

5. Dendritic cell-targeted nanoparticles for the delivery of DNA and protein vaccines. [D] . Raghuwanshi, Dharmendra. 2012

机译：树突状细胞靶向纳米颗粒，用于递送DNA和蛋白质疫苗。

6. The Order of Prime-Boost Vaccination of Neonatal Calves with Mycobacterium bovis BCG and a DNA Vaccine Encoding Mycobacterial Proteins Hsp65 Hsp70 and Apa Is Not Critical for Enhancing Protection against Bovine Tuberculosis [O] . Margot A. Skinner, D. Neil Wedlock, Geoffrey W. de Lisle, 2005

机译：用牛分枝杆菌BCG和编码分枝杆菌蛋白Hsp65Hsp70和Apa的DNA疫苗对初生牛犊进行初次加强免疫接种的顺序对于增强对牛结核病的保护作用并不关键

7. The Order of Prime-Boost Vaccination of Neonatal Calves with Mycobacterium bovis BCG and a DNA Vaccine Encoding Mycobacterial Proteins Hsp65, Hsp70, and Apa Is Not Critical for Enhancing Protection against Bovine Tuberculosis [O] . Skinner, Margot A., Wedlock, D. Neil, de Lisle, Geoffrey W., 2005

机译：用牛分枝杆菌卡介苗和编码分枝杆菌蛋白Hsp65，Hsp70和Apa的DNA疫苗对初生牛犊进行初次加强免疫接种的顺序对于增强对牛结核病的保护并不关键

1. 结核分枝杆菌休眠相关抗原Rv1733c DNA疫苗的构建及免疫学特性研究 [J] . 段安虎 ,张薇 ,柏银兰 . 中国防痨杂志 . 2013,第009期

2. 编码破伤风毒素C片段(TetC)的DNA疫苗初免及TetC粘膜加强的免疫策略 [J] . 边国林 . 国外医学：预防．诊断．治疗用生物制品分册 . 2003,第2期

3. 传统 BCG 初免 IL-12联合 Ag85A DNA 疫苗加强序贯免疫小鼠效果观察 [J] . 王婵 ,王新敏 ,季榕 . 中国人兽共患病学报 . 2014,第010期

4. 抗旋毛虫病Ts87 DNA疫苗滴鼻初免—蛋白疫苗加强免疫策略增强免疫保护应答 [J] . 顾园 ,黄京京 ,杨静 . 寄生虫与医学昆虫学报 . 2016,第002期

5. 超抗原SEA增强小鼠对HBV DNA疫苗的免疫反应 [J] . 靳彦文 ,李平 ,胥全彬 . 生物工程学报 . 2005,第005期

6. DNA疫苗加强免疫显著提高卡介苗初免小鼠的抗结核T细胞免疫反应及其保护效力 [C] . 范小勇 ,康涵 ,胡志东 . 中华医学会结核病学分会2014年学术大会 . 2014

7. 乙肝表面抗原DNA疫苗初免—蛋白疫苗加强免疫策略增强免疫反应的蛋白质组学分析 [A] . 李德安 . 2006

1. 编码诊断相关的病毒衣壳抗原的EB病毒DNA序列,通过聚合酶链反应产生的表达克隆和该重组抗原在诊断检测中的应用 [P] . 中国专利： CN1117150C . 2003.08.06

2. 编码诊断相关的病毒衣壳抗原的EB病毒DNA序列，通过聚合酶链反应产生的表达克隆和该重组抗原在诊断检测中的应用 [P] . 中国专利： CN1129460A . 1996-08-21

3. POLYPEPTIDE CONTAINING ANTIGENIC PART OF SOLUBLE ANTIGEN M. TUBERCULOSIS OR VARIANT OF SAID ANTIGEN, DNA MOLECULE ENCODING SUCH POLYPEPTIDE, EXPRESSION VECTOR, HOST CELL, DIAGNOSTIC METHODS OF M. TUBERCULOSIS INFECTION AND DIAGNOSTIC KITS [P] . 外国专利： CZ9901266A3 . 1999-09-15

机译：含有可溶性抗原的结核分枝杆菌的抗原部分或结核分枝杆菌的抗原，编码此类多肽的DNA分子，表达载体，宿主细胞，结核分枝杆菌感染的诊断方法和诊断试剂盒

4. POLYPEPTIDE CONTAINING ANTIGENIC PART OF SOLUBLE ANTIGEN M. TUBERCULOSIS OR VARIANT OF SAID ANTIGEN, DNA MOLECULE ENCODING SUCH POLYPEPTIDE, EXPRESSION VECTOR, HOST CELL, DIAGNOSTIC METHODS OF M. TUBERCULOSIS INFECTION AND DIAGNOSTIC KITS [P] . 外国专利： CZ126699A3 . 1999-09-15

机译：含有可溶性抗原的结核分枝杆菌的抗原部分或结核分枝杆菌的抗原，编码此类多肽的DNA分子，表达载体，宿主细胞，结核分枝杆菌感染的诊断方法和诊断试剂盒

5. Polypeptides comprising a portion of an immunogenic Antigen of M.Tuberculosis soluble, and DNA molecules encoding such polypeptides, expression vector Resistance comprising Said DNA Molecules, host cells transformed with these vectors.The pharmaceutical compositions. [P] . 外国专利： AR018100A1 . 2001-10-31

机译：包含一部分结核分枝杆菌免疫原性抗原的多肽和编码此类多肽的DNA分子，包含所述DNA分子的表达载体抗性，用这些载体转化的宿主细胞。药物组合物。

相关主题

Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice.

摘要

著录项

相似文献

相关主题

期刊订阅