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首页> 外文期刊>Biomaterials Science >Decreased tissue factor expression with increased CD11b upregulation on elastin-based biomaterial coatings
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Decreased tissue factor expression with increased CD11b upregulation on elastin-based biomaterial coatings

机译：基于弹性蛋白的生物材料涂层上的组织因子表达降低，CD11b表达上调

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摘要

Elastin-like polypeptide (ELP) coatings have been shown to have non-thrombogenic properties both in vitro and in vivo. In this work, we expand our understanding of this phenomenon by investigating the interaction of these coatings with leukocytes. Citrated whole blood was exposed to a shear rate of 300 s~(-1) for 2 hours at 37 ℃ on ELP1- and ELP4-coated polyethylene terephthalate (Mylar) surfaces in a cone and plate device. Scanning electron microscopy and flow cytometry were used to measure leukocyte activation and platelet-leukocyte aggregation in response to the ELP1 and ELP4 coatings on the surface and in the bulk, respectively. Surface analysis showed little leukocyte activity on the surface of uncoated positive controls. Both the tissue factor (TF) expression (indicative of leukocyte activation) and CD61 expression (indicative of platelet-leukocyte aggregates), in the bulk were decreased by 40% and 20%, respectively, with the ELP coating of Mylar, while a two- to three-fold increase in CD11b upregulation (indicative of leukocyte activation) for ELP1 and ELP4 was determined. Two of three bulk markers indicated that ELP-coated Mylar decreased the leukocyte response compared to the uncoated Mylar, while the third, CD11b, indicated an increase in leukocyte response to the ELP coatings.
机译：弹性蛋白样多肽（ELP）涂层在体外和体内均具有非血栓形成特性。在这项工作中，我们通过研究这些涂层与白细胞的相互作用来扩展对这种现象的理解。在锥板装置中，将柠檬酸化的全血在37℃下于300 s〜（-1）的剪切速率下在ELP1和ELP4涂覆的聚对苯二甲酸乙二醇酯（Mylar）表面上暴露2个小时。扫描电子显微镜和流式细胞仪分别用于测量白细胞的活化和血小板-白细胞的聚集，以分别响应表面和整个表面上的ELP1和ELP4涂层。表面分析显示未涂覆的阳性对照表面上的白细胞活性很小。使用Mylar的ELP涂层时，组织中的组织因子（TF）表达（指示白细胞激活）和CD61表达（指示血小板-白细胞聚集）分别降低了40％和20％。 -确定了ELP1和ELP4的CD11b上调（指示白细胞激活）增加了三倍。三种大块标记中的两种表示与未涂覆的聚酯薄膜相比，ELP涂覆的聚酯薄膜降低了白细胞反应，而第三种CD11b则表明对ELP涂层的白细胞响应增加。

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