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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Selective Extraction and Isolation of Vitamin B12 Using Homogeneous Liquid-Liquid Extraction with Perfluoro Surfactant
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Selective Extraction and Isolation of Vitamin B12 Using Homogeneous Liquid-Liquid Extraction with Perfluoro Surfactant

机译：全氟表面活性剂均相液体萃取选择性萃取和分离维生素B12

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摘要

Cyanocobalamin(vitamin B12)was selectively extracted from a biological sample by the homogeneous liquid-liquid extraction based on a pH-dependent phase separation with perfluorocctanoate ion(PFOA~-).When the experimental conditions were [PFOA~-]_T=2.86X10~-3 mol L~-1,[HCl]_T=0.66 mol L~-1,and[Acetone]_T=2.86 vol %,8.46X10~-7 mol L~-1(1.14 ppm)of vitamin B_12 was selectively extracted at 95.2%(sample solution;35Mlàa liquid sedimented phase;30 muL).This liquid sedimented phase consisted of large amounts of HPFOA.When microliter levels of THF and water were added to this sedimented phase,vitamin B_12 is completely and rapidly back-extracted from the sedimented phase to the water phase.Vitamin B_12 was then extracted into the water phase at 96.2% and all DPFOA remained in the sedimented phase.The rest of the sedimented phase after the back-extraction was dried and completely recovered as a powder of HPFOA.Consequently,this method was able to recycle HPFOA as a separation medium.Vitamin B_12 in a water phase was also crystallized by the addition of excess THF.Moreover,this method was applied to the extraction from rare oysters.As a result,a few micrograms of vitamin B_12 were recovered.
机译：在全氟辛酸根离子（PFOA〜-）的pH依赖性相分离的基础上，通过均相液液萃取，从生物样品中选择性提取氰钴胺（维生素B12）。当实验条件为[PFOA〜-] _ T = 2.86X10时〜-3 mol L〜-1，[HCl] _T = 0.66 mol L〜-1，和[丙酮] _T = 2.86 vol％，选择性地选择了维生素B_12的8.46X10〜-7 mol L〜-1（1.14 ppm）以95.2％的浓度萃取（样品溶液; 35毫升液体沉淀相; 30微升）。该液体沉淀相由大量HPFOA组成。当向该沉淀相中加入微升四氢呋喃和水时，维生素B_12会被完全，快速地反萃取。从沉淀相萃取到水相，然后将维生素B_12以96.2％的比例萃取到水相中，所有DPFOA都保留在沉淀相中，反萃取后的其余沉淀相被干燥并以粉末形式完全回收因此，该方法能够回收HPFOA作为分离介质。维生素B_1通过加入过量的THF也使2相在水相中结晶。此外，该方法还用于从稀有牡蛎中提取。结果回收了几微克维生素B_12。

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