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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Electronic Properties and Thermal Stability of Soluble Redox Proteins from a Thermophilic Purple Sulfur Photosynthetic Bacterium,Thermochromatium tepidum
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Electronic Properties and Thermal Stability of Soluble Redox Proteins from a Thermophilic Purple Sulfur Photosynthetic Bacterium,Thermochromatium tepidum

机译：嗜热紫色硫光合细菌热生色菌嗜热色氨酸的可溶性氧化还原蛋白的电子性质和热稳定性

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摘要

The electronic properties of soluble redox proteins from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium(T.)tepidum have been studied in terms of electronic absorption,magnetic CD,midpoint redox potential,and electron-transfer behavior.T.tepidum possesses one high potential iron-sulfur protein(HiPIP),one cyto-chrome c',and two low potential cytochrome c-552's.The two low potential cytochrome c-552's can be separated further into a higher and a lower potential species.The HiPIP has a midpoint redox potential of +340 mV at pH 7.From electron-transfer ability shown by flash experiments,the HiPIP was confirmed to be the possible electron-transfer protein to the reaction center under physiological conditions.The HiPIP is thermally stable up to 60 deg C,which is much higher than that of the mesophilic photosynthetic bacterium,Allochromatium(A.)vinosum.Cytochrome c' has a high-spin pro-toheme with a redox midpoint potential of +100 mV at pH 7,similar to those of many oth'er photosynthetic bacteria.The molecular weight was determined to be 14147 Da by MALDI-TOF/MS experiments.The higher potential cytochrome c-552' s have hemes c's with midpoint redox potentials of - 15 and +85 mV,and the lower one of - 15 mV.The molecular weights for the higher potential cytochrome c-552 and the lower potential c-552 were found to be 20702 and 47757 Da,respectively.The higher potential cytochrome c-552 has an attached flavoprotein with a molecular mass of 42000 Da.The lower potential cytochrome c-552 has been found for the first time in T.tepidum.
机译：从电子吸收，磁性CD，中点氧化还原电势和电子转移行为等方面研究了嗜热紫硫光合细菌嗜热铬（T.）的可溶性氧化还原蛋白的电子性质。 -硫蛋白（HiPIP），一个细胞色素c'和两个低电势细胞色素c-552's。两个低电势细胞色素c-552's可以进一步分离为高电势和低电势物种.HiPIP具有中点氧化还原pH值为+340 mV时，电位为+340 mV。根据快速实验显示的电子转移能力，证实了HiPIP是在生理条件下可能将电子转移至反应中心的蛋白质.HiPIP在高达60摄氏度的温度下具有热稳定性，细胞色素c'具有高自旋原血红素，在pH 7下的氧化还原中点电势为+100 mV，与许多其他光合细菌相比，具有更高的自旋血红素原。的光合作用细菌。通过MALDI-TOF / MS实验确定分子量为14147 Da。较高电势的细胞色素c-552's具有血红素c's，中点氧化还原电势为-15和+85 mV，而较低15 mV之一。较高电位的细胞色素c-552和较低电位的c-552的分子量分别为20702和47757 Da。较高电位的细胞色素c-552具有附着的黄素蛋白，其分子量为潜在的细胞色素c-552的首次发现是在T.tepidum中，它的分子量为42000 Da。

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