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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Characterization of Non-Native Heme Coordination Structures Emerging upon Guanidine Hydrochloric Acid-Induced Unfolding of Pseudomonas aeruginosa Ferricytochrome c_(551)
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Characterization of Non-Native Heme Coordination Structures Emerging upon Guanidine Hydrochloric Acid-Induced Unfolding of Pseudomonas aeruginosa Ferricytochrome c_(551)

机译：盐酸胍诱导的铜绿假单胞菌铁细胞色素c_（551）的折叠出现的非天然血红素配位结构的表征。

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摘要

The non-native heme coordination structures emerging upon guanidine hydrochloric acid(GdnHCl)-induced unfolding of Pseudomonas aeruginosa ferricytochrome c_(551)were characterized by absorption,circular dichroism,paramagnetic NMR,and resonance Raman spectroscopy.Hexacoordinated high-spin ferriheme bearing axial His and H_2O ligands,and pentacoordinated high-spin ferriheme with an axial His ligand were identified in the presence of GdnHCl concentrations >= 1.5 M(M = mol dm~(-3)).These non-native species were rapidly interconverted to each other through cleavage/formation of the Fe-H_2O coordination bond,and were also in dynamic equilibrium,at a rate of < 2 X 10~4 s~(-1),with the native species.Thus,the present study demonstrated the presence of dynamic equilibrium through cleavage/formation of a Fe-H_2O coordination bond in an unfolding intermediate.These results provide a deeper insight into structure transitions of the heme active site upon folding and unfolding of cytochrome c.
机译：通过吸收，圆二色性，顺磁核磁共振和共振拉曼光谱表征了盐酸胍（GdnHCl）诱导的铜绿假单胞菌铁细胞色素c_（551）解折叠时出现的非天然血红素配位结构。在GdnHCl浓度> = 1.5 M（M = mol dm〜（-3））的条件下，鉴定了具有轴向His配体的五配位高自旋亚铁血红素。这些非天然物种彼此之间可以快速相互转化。通过与Fe-H_2O配位键的裂解/形成，并且还与天然物种处于动态平衡，速率<2 X 10〜4 s〜（-1）。因此，本研究证明了存在通过在未折叠的中间体中裂解/形成Fe-H_2O配位键来实现动态平衡。这些结果为细胞色素c折叠和展开时血红素活性位点的结构转变提供了更深入的了解。

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