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首页> 外文期刊>The British Journal of Nutrition >The effect of marine oil-derived n-3 fatty acids on transepithelial calcium transport in Caco-2 cell models of healthy and inflamed intestines.
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The effect of marine oil-derived n-3 fatty acids on transepithelial calcium transport in Caco-2 cell models of healthy and inflamed intestines.

机译：在健康和发炎的肠道的Caco-2细胞模型中，海洋油衍生的n-3脂肪酸对上皮钙转运的影响。

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Marine oil-derived n-3 fatty acids have been shown to stimulate intestinal Ca absorption in animal studies, but the effects of such fatty acids on Ca absorption in human subjects are relatively unknown. In particular, n-3 fatty acids may be of therapeutic value for some Crohn's disease patients who experience Ca malabsorption. Therefore, the aim of the present study was to investigate the effect of 20 : 5n-3 and 22 : 6n-3 on transepithelial Ca transport across monolayers of healthy Caco-2 cells as well as of TNF-alpha-treated Caco-2 cells (an in vitro model of Crohn's disease). Caco-2 cells were seeded onto permeable filter supports and allowed to differentiate into monolayers, which were treated with 80 microM-20 : 5n-3, 80 microM-22 : 6n-3, or 40 microM-20 : 5n-3 + 40 microM-22 : 6n-3 for 6 or 8 d, with or without co-treatment with TNF-alpha (10 ng/ml) (n 11-15 monolayers per treatment). On day 16, transepithelial and transcellular transport of 45Ca and fluorescein transport (a marker of paracellulardiffusion) were measured. Treatment of healthy and inflamed Caco-2 cells with 20 : 5n-3, 22 : 6n-3 and both fatty acids combined for 8 d significantly (P < 0.005-0.01) increased total transepithelial Ca transport compared with that in control, effects which were mediated by an enhanced rate of transcellular Ca transport. The effects of n-3 fatty acids on Ca absorption after 6 d were less clear-cut. In conclusion, the present in vitro findings highlight the need to investigate the effect of marine oil-based n-3 fatty acids on Ca absorption in vivo in studies of healthy human subjects as well as of Crohn's disease patients.
机译：在动物研究中，海洋油衍生的n-3脂肪酸可刺激肠道Ca的吸收，但是这种脂肪酸对人类受试者Ca吸收的影响相对未知。特别是，n-3脂肪酸对某些吸收钙吸收不良的克罗恩氏病患者可能具有治疗价值。因此，本研究的目的是研究20：5n-3和22：6n-3对跨健康Caco-2细胞以及TNF-α处理的Caco-2细胞单层跨上皮Ca转运的影响（克罗恩病的体外模型）。将Caco-2细胞接种到可渗透的滤膜载体上，使其分化为单层，然后用80 microM-20：5n-3、80 microM-22：6n-3或40 microM-20：5n-3 + 40处理microM-22：6n-3持续6或8 d，有或没有与TNF-alpha（10 ng / ml）共同处理（每次处理n 11-15个单层）。在第16天，测量了45Ca的上皮和跨细胞运输以及荧光素运输（细胞旁扩散的标志）。与对照组相比，用20：5n-3、22：6n-3和两种脂肪酸联合治疗健康和发炎的Caco-2细胞显着提高了8 d（P <0.005-0.01），这两种脂肪酸的作用均较对照组高。通过增加的跨细胞钙运输速率介导。 6天后n-3脂肪酸对Ca吸收的影响不那么明确。总之，目前的体外研究结果表明，有必要在健康人类受试者以及克罗恩氏病患者的研究中研究海洋油基n-3脂肪酸对体内Ca吸收的影响。

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