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首页> 外文期刊>The British Journal of Nutrition >Lactobacillus rhamnosus and its cell-free culture supernatant differentially modulate inflammatory biomarkers in Escherichia coli-challenged human dendritic cells.
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Lactobacillus rhamnosus and its cell-free culture supernatant differentially modulate inflammatory biomarkers in Escherichia coli-challenged human dendritic cells.

机译：鼠李糖乳杆菌及其无细胞培养物上清液可不同地调节大肠杆菌攻击的人树突状细胞中的炎症生物标志物。

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The intestinal immune system maintains a delicate balance between immunogenicity against invading pathogens and tolerance to the commensal microbiota and food antigens. Different strains of probiotics possess the ability to finely regulate the activation of dendritic cells (DC), polarising the subsequent activity of T-cells. Nevertheless, information about their underlying mechanisms of action is scarce. In the present study, we investigated the immunomodulatory effects of a potentially probiotic strain, Lactobacillus rhamnosus CNCM I-4036, and its cell-free culture supernatant (CFS) on human DC challenged with Escherichia coli. The results showed that the levels of pro-inflammatory cytokines such as IL-1 beta , IL-6, IL-8 and IL-12p70 were higher in the cells treated with live L. rhamnosus than in the cells treated with the CFS. In the presence of E. coli, the supernatant was more effective than the probiotic bacteria in reducing the secretion of pro-inflammatory cytokines. In addition, live L. rhamnosus potently induced the production of transforming growth factor (TGF)- beta 1 and TGF- beta 2, whereas the CFS increased the secretion of TGF- beta 1. However, in the presence of E. coli, both treatments restored the levels of TGF- beta . The probiotic strain L. rhamnosus CNCM I-4036 and its CFS were able to activate the Toll-like receptor signalling pathway, enhancing innate immunity. The two treatments induced gene transcription of TLR-9. Live L. rhamnosus activated the expression of TLR-2 and TLR-4 genes, whereas the CFS increased the expression of TLR-1 and TLR-5 genes. In response to the stimulation with probiotic/CFS and E. coli, the expression of each gene tested was notably increased, with the exception of TNF- alpha and NFKBIA. In conclusion, the CFS exhibited an extraordinary ability to suppress the production of pro-inflammatory cytokines by DC, and may be used as an effective and safer alternative to live bacteria.
机译：肠道免疫系统在抵抗入侵病原体的免疫原性与对共生微生物和食物抗原的耐受性之间保持着微妙的平衡。益生菌的不同菌株具有精细调节树突状细胞（DC）活化的能力，可极化随后的T细胞活性。然而，关于其潜在作用机制的信息很少。在本研究中，我们调查了潜在的益生菌菌株鼠李糖乳杆菌CNCM I-4036及其无细胞培养上清液（CFS）对受大肠杆菌攻击的人DC的免疫调节作用。结果表明，用活鼠李糖乳杆菌处理的细胞中的促炎细胞因子如IL-1β，IL-6，IL-8和IL-12p70的水平高于用CFS处理的细胞。在大肠杆菌的存在下，上清液在减少促炎细胞因子分泌方面比益生菌更有效。此外，活的鼠李糖乳杆菌有效诱导了转化生长因子（TGF）-β1和TGF-β2的产生，而CFS则增加了TGF-β1的分泌。但是，在存在大肠杆菌的情况下，两者治疗恢复了TGF-β的水平。益生菌株鼠李糖乳杆菌CNCM I-4036及其CFS能够激活Toll样受体信号传导途径，增强先天免疫力。两种处理诱导TLR-9的基因转录。活的鼠李糖乳杆菌激活了TLR-2和TLR-4基因的表达，而CFS增加了TLR-1和TLR-5基因的表达。响应于益生菌/ CFS和大肠杆菌的刺激，除TNF-α和NFKBIA外，每个测试基因的表达均显着增加。总之，CFS具有抑制DC促炎性细胞因子产生的非凡能力，可以用作活细菌的有效和安全替代方法。

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