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首页> 外文期刊>The Indian Journal of Animal Reproduction >BUFFALO SPERMATOZOA CLASSIFIED BASED ON PLASMALEMMA FUNCTIONAL INTEGRITY AND ACROSOMAL INTEGRITY TESTS
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BUFFALO SPERMATOZOA CLASSIFIED BASED ON PLASMALEMMA FUNCTIONAL INTEGRITY AND ACROSOMAL INTEGRITY TESTS

机译：基于等离子功能完整性和原子完整性测试的水牛精子分类

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The aim of this study was to assess the plasmalemma functional integrity and acrosomal integrity and their relationship with sperm functional tests in assessing buffalo semen quality. Fifty-two ejaculates from thirteen Murrah buffalo bulls were used for the study. Plasmalemma integrity and acrosomal integrity was assessed by fluorogenic and hypoosmotic swelling- Giemsa (HOS-G) test. The samples were classified into different subpopulations based on their reaction to fluorogenic staining and HOS-G test, respectively. No difference in plasmalemma integrity (%) was observed (49.11 ±2.53 vs. 48.59±2.48) between fluorogenic and HOS-G tests. However, the acrosomal integrity (%) assessed by HOS-G test (82.43±4.15) was significantly higher (P<0.05) thanfluorogenic stain (68.12±3.03). The sperm subpopulation (%) positive for plasmalemma integrity and acrosomal integrity (P+A+) was significantly (P<0.05) higher in fluorogenic (49.11 ±2.53) than HOS-G (43.08±2.38) tests. Further, the plasmalemma functional membrane integrity (48.59±2.48 %) was significantly higher than the sperm P~+A~+ subpopulation (43.08±2.38 %). In the P~+A~+subpopulation, significant correlation (r= 0.529, P<0.05) was observed between fluorogenic and HOS-G test. The sperm P~+A~+subpopulation had a significant positive correlation with the progressive forward motility (r=0.733, fluorogenic; r=0.666, HOS-G), acrosomal integrity (r=0.844, fluorogenic; r=0.569, HOS-G) and sperm nuclear morphology (r=0.617, fluorogenic; r=0.752, HOS-G). The study confirmed that fluorogenic and HOS-G tests measure the functional membrane integrity. It can be concluded that the assessing of sperm P+A+ subpopulation by HOS-G test is easy and simple and can be recommended as a routine test to evaluatebuffalo semen quality.
机译：这项研究的目的是评估水牛精液质量的质膜功能完整性和顶体完整性及其与精子功能测试的关系。这项研究使用了13头Murrah水牛公牛的52例射精。通过荧光和低渗溶胀-吉姆萨（HOS-G）试验评估血浆雌激素完整性和顶体完整性。根据样品对荧光染色的反应和HOS-G测试，将样品分为不同的亚群。在荧光测试和HOS-G测试之间未观察到血浆缺陷完整性（％）的差异（49.11±2.53对48.59±2.48）。然而，通过HOS-G测试评估的顶体完整性（％）（82.43±4.15）显着高于荧光染色（68.12±3.03）（P <0.05）。在荧光检查（49.11±2.53）中，血浆缺陷完整性和顶体完整性（P + A +）阳性的精子亚群（％）显着（P <0.05）比HOS-G（43.08±2.38）测试高。此外，质膜功能膜完整性（48.59±2.48％）明显高于精子P〜+ A〜+亚群（43.08±2.38％）。在P〜+ A〜+亚群中，荧光检测与HOS-G检测之间存在显着相关性（r = 0.529，P <0.05）。精子P〜+ A〜+亚群与进行性向前运动（r = 0.733，荧光; r = 0.666，HOS-G），顶体完整性（r = 0.844，荧光; r = 0.569，HOS- G）和精子的核形态（r = 0.617，荧光; r = 0.752，HOS-G）。该研究证实，荧光检测和HOS-G检测可以测量功能性膜的完整性。可以得出结论，通过HOS-G测试评估精子P + A +亚群是容易和简单的，可以推荐作为评估水牛精液质量的常规测试。

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