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首页> 外文期刊>The Indian Journal of Animal Sciences >Determination of male-specific gene expression of White Lamphun cattle semen treated by monoclonal antibodies
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Determination of male-specific gene expression of White Lamphun cattle semen treated by monoclonal antibodies

机译：单克隆抗体处理白喃奔牛精液雄性基因表达的测定

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In the present study, male-specific monoclonal antibodies, P1C2B8 and P1C2C9, were used for sperm sexing via agglutination and cytotoxic reactions in White Lamphun cattle semen no. 862, no. 863 and no. KL. Live sperm percentage was used to find out an optimum dilution of antibody in agglutination and cytotoxic reactions and multiplex real time polymerase chain reaction was used to determine male-specific gene expression in each optimum dilution. In agglutination treatment, the results showed that all cattle had the same optimum antibody dilution while in cytotoxicity treatment, each cattle had different optimum antibody dilution. The results from multiplex real time polymerase chain reaction also showed that in both agglutination and cytotoxicity treatments, male specific gene expression in semen samples was lower in cattle no. 862, no. 863 and no. KL when compared to control treatment. From these results, it was implied that antibodies P1C2B8 and P1C2C9 could alter the sperm sex ratio by reducing Y sperm in White Lamphun cattle semen.
机译：在本研究中，雄性特异性单克隆抗体P1C2B8和P1C2C9通过凝集和细胞毒性反应在南邦府白色牛精液中用于精子性别鉴定。 862号863号吉隆坡使用活精子百分比在凝集和细胞毒性反应中找到抗体的最佳稀释度，并使用多重实时聚合酶链反应确定每种最佳稀释度中男性特异性基因的表达。在凝集处理中，结果显示所有牛的最佳抗体稀释度均相同，而在细胞毒性处理中，每只牛的最佳抗体稀释度均不同。多重实时聚合酶链反应的结果还显示，在凝集和细胞毒性处理中，牛的精液样品中男性特异性基因表达均较低。 862号863号与对照治疗相比，KL。从这些结果暗示，抗体P1C2B8和P1C2C9可以通过减少白喃奔牛精液中的Y精子来改变精子性别比。

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