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首页> 外文期刊>The Canadian Journal of Neurological Sciences: le Journal Canadien des Sciences Neurologiques >Expression of Urotensin II During Focal Cerebral Ischemic in Diabetic Rats
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Expression of Urotensin II During Focal Cerebral Ischemic in Diabetic Rats

机译：糖尿病大鼠局灶性脑缺血过程中尿降压素II的表达

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摘要

Background: The objective of this study was to explore the expression of urotensin II (UII), its receptor (GPR14), and vascular endothelial growth factor (VEGF), as well as their associations in the ischaemic brains of rats with focal cerebral ischaemia, under normal and diabetic conditions. Methods: Diabetes mellitus (DM) was induced by injection of streptozotocin (STZ) into Sprague-Dawley rats. Focal cerebral ischaemia was induced by middle cerebral artery occlusion (MCAO) four weeks after DM onset by STZ. Rats (n=80) were divided into four groups: normal control, DM, MCAO, and DM/MCAO. Immunohistochemistry and reverse-transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expression of UII. GPR14 and VEGF in the diabetic and ischaemic brain. Results: Expression of UII and GPR 14 was increased at mRNA and protein levels in the DM and MCAO group compared with controls. In the DM/MCAO group, expression of UII and GPR14 was increased significantly in the ischaemic brain, and was accompanied by a significantly increased VEGF expression. Conclusion: Diabetes mellitus was seen to aggravate brain lesions after ischaemia, and UII may have an important role.
机译：背景：本研究的目的是探讨尿紧张素II（UII），其受体（GPR14）和血管内皮生长因子（VEGF）的表达，以及它们在局灶性脑缺血大鼠缺血性脑中的相关性，在正常和糖尿病情况下。方法：通过将链脲佐菌素（STZ）注射到Sprague-Dawley大鼠中来诱导糖尿病（DM）。 STZ DM发病4周后，大脑中动脉闭塞（MCAO）引起局灶性脑缺血。将大鼠（n = 80）分为四组：正常对照组，DM，MCAO和DM / MCAO。免疫组织化学和逆转录聚合酶链反应（RT-PCR）用于检测UII的表达。糖尿病和缺血性脑中的GPR14和VEGF。结果：与对照组相比，DM和MCAO组的UII和GPR 14表达在mRNA和蛋白质水平上增加。在DM / MCAO组中，UII和GPR14的表达在缺血性脑中显着增加，并伴有VEGF表达的显着增加。结论：缺血后糖尿病会加剧脑损伤，UII可能起重要作用。

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