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首页> 外文期刊>The international journal of tuberculosis and lung disease: the official journal of the International Union against Tuberculosis and Lung Disease >Antibody response to culture filtrate antigens of Mycobacterium tuberculosis during and after treatment of tuberculosis patients.
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Antibody response to culture filtrate antigens of Mycobacterium tuberculosis during and after treatment of tuberculosis patients.

机译：结核病患者治疗期间和治疗后对结核分枝杆菌培养滤液抗原的抗体反应。

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SETTING: Many authors have shown rising titres of anti-mycobacterial antibodies after a few months of anti-tuberculosis treatment. This humoral response might persist for years, making the discrimination between current and old disease difficult. OBJECTIVE: Characterisation of the humoral response to culture filtrates of Mycobacterium tuberculosis before and after treatment of tuberculous patients in order to identify those antigens that could provide information about disease activity. METHODS: Anti-mycobacterial IgG response was determined during and after treatment of tuberculous patients by ELISA and immunoblot. Serum was taken from 71 active tuberculous patients (59 newly acquired and 12 relapse), 15 old tuberculous patients and 45 nontuberculous control subjects. RESULTS: By ELISA, antibody response increased after 2 months of treatment. After chemotherapy was completed, the estimated number of antibodies remained at the same level. The level of specific antibodies in patients seems to reach the same level as that of control subjects 3 years after initiation of treatment. In Western blot, although each patient serum had its own characteristic banding pattern, differences between tuberculous patients and control subjects were found in the area below 20 kDa. Serum from tuberculous patients showed high levels of antibodies at the 14 kDa region. After the beginning of treatment, the intensity of the 14 kDa region band and the percentage of positive recognition tended to decrease. Therefore, one year after initiation of treatment, only seven of 13 cases who demonstrated anti-mycobacterial antibodies in ELISA revealed a mild but still positive reaction at the 14 kDa region; this reactivity disappeared 2 years after initiation of chemotherapy. CONCLUSIONS: The 14 kDa region antigen seems to induce a humoral response that evolves in relation with the disease activity.
机译：地点：经过数月的抗结核治疗后，许多作者已经显示出抗分枝杆菌抗体的滴度不断上升。这种体液反应可能会持续数年，因此很难区分当前和旧疾病。目的：对结核病患者治疗前后结核分枝杆菌培养滤液的体液反应进行表征，以鉴定可提供有关疾病活动信息的抗原。方法：通过ELISA和免疫印迹法测定结核病患者治疗期间和治疗后的抗分枝杆菌IgG反应。从71名活动性结核病患者（59名新获得的结核病和12名复发性结核病），15名老年结核病患者和45名非结核病对照受试者中获取血清。结果：通过ELISA，治疗2个月后抗体应答增加。化疗完成后，估计的抗体数量保持在相同水平。开始治疗后3年，患者体内的特异性抗体水平似乎达到了与对照组相同的水平。在蛋白质印迹法中，尽管每种患者的血清都有其自己的特征性条带模式，但在20 kDa以下的区域发现了结核病患者与对照组之间的差异。结核病患者的血清在14 kDa区域显示出高水平的抗体。开始治疗后，14 kDa区域带的强度和阳性识别的百分比趋于下降。因此，在开始治疗的一年后，在ELISA中显示抗分枝杆菌抗体的13例病例中，只有7例在14 kDa区域显示出轻度但仍呈阳性反应。在开始化疗后2年，这种反应性消失了。结论：14 kDa区域抗原似乎诱导了与疾病活动有关的体液反应。

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