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首页> 外文期刊>The international journal of biochemistry and cell biology >Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells.
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Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells.

机译：人血管微血管内皮细胞中微血管内皮细胞的起源。

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摘要

In microvessels, periendothelial cells expressing alpha smooth muscle actin (alphaSMA) interact with the endothelial cells and are essential for vessel maturation and stabilization. In adult tissues, the cellular origin of the periendothelial cells is still not clear, in particular in humans. To determine the origin of human periendothelial cells, we used a recently developed 3D co-culture system that mimics human skin connective tissue. This system is composed of normal human dermal fibroblasts (NHDF), human dermal microvascular endothelial cells (HMEC-1), and a collagen matrix. In this system, "microvessels" composed of an endothelial lumen associated with periendothelial cells develop. Using this co-culture system, we (i) labelled fibroblasts with the vital dye CFDA-SE, cultured them with unlabelled endothelial cells, and observed that only endothelium-associated CFDA-SE-labelled cells express alphaSMA; (ii) infected endothelial cells with a retrovirus stably expressing eGFP, cultured them with unlabelled fibroblasts, and observed that cells expressing alphaSMA did not co-express eGFP, but were associated with the eGFP-expressing endothelial cells of the microvessels. Together, these results indicate that periendothelial cells arise by differentiation from fibroblasts and that they require interaction with endothelial cells to do so.
机译：在微血管中，表达α平滑肌肌动蛋白（alphaSMA）的内皮细胞与内皮细胞相互作用，对于血管成熟和稳定至关重要。在成人组织中，内皮细胞的细胞起源仍然不清楚，特别是在人类中。为了确定人类内皮细胞的来源，我们使用了最近开发的模仿人类皮肤结缔组织的3D共培养系统。该系统由正常人真皮成纤维细胞（NHDF），人真皮微血管内皮细胞（HMEC-1）和胶原蛋白基质组成。在该系统中，形成了由与内皮细胞相关的内皮管腔构成的“微血管”。使用这种共培养系统，我们（i）用活性染料CFDA-SE标记成纤维细胞，并用未标记的内皮细胞培养它们，并观察到只有内皮相关的CFDA-SE标记的细胞表达alphaSMA; （ii）用稳定表达eGFP的逆转录病毒感染的内皮细胞，用未标记的成纤维细胞培养它们，并观察到表达αSMA的细胞不共表达eGFP，但与微血管表达eGFP的内皮细胞有关。总之，这些结果表明内皮细胞通过成纤维细胞的分化而产生，并且它们需要与内皮细胞相互作用。

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