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首页> 外文期刊>The Japanese Journal of Veterinary Research >Experimental transmission of Bovine leukemia virus in cattle via rectal palpation
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Experimental transmission of Bovine leukemia virus in cattle via rectal palpation

机译：经直肠触诊牛牛白血病病毒的实验性传播

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摘要

We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV-negative steers. Three of four steers developed antibodies against BLV as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experimentsdemonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection.
机译：我们检查了牛白血病病毒（BLV）是否通过在BLV感染的牛和BLV阴性ste牛之间使用共同的套管通过触诊传播。在第一次直肠触诊后的7至10周内，使用感染BLV的母牛的普通袖子，通过琼脂凝胶免疫扩散（AGID）试验确定，四分之三的牛中发展了针对BLV的抗体。在the牛中，通过PCR检测到BLV前病毒DNA比通过AGID测试检测抗体早1至5周。我们的实验表明，直肠触诊是牛群中BLV传播的潜在原因，并且通过PCR检测牛中BLV前病毒DNA对于早期诊断BLV感染是有用的筛选测试。

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