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首页> 外文期刊>The Japanese Journal of Veterinary Research >Analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant Babesia gibsoni isolate in vitro
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Analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant Babesia gibsoni isolate in vitro

机译：耐地亚那莫的乙酸巴贝斯长臂猿离体菌株的能量产生和糖酵解途径分析

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摘要

In our previous study, the level of parasitemia of the diminazene aceturate (DA)-resistant B. gibsoni isolate was continuously lower than that of the wild-type, indicating the possible alteration of energy metabolism in that isolate. Therefore, in the present study, the concentrations of ATP, glucose, lactate, and pyruvate, and the activities of lactate dehydrogenase and pyruvate kinase in the wild-type and DA-resistant isolate of B. gibsoni were measured and compared to investigate the amount of energy generation and the activity of the glycolysis pathway. As a result, the intracellular ATP and glucose concentrations in the DA-resistant B. gibsoni isolate were significantly higher than those in the wild-type. Meanwhile, the concentrations of lactate and pyruvate and the activities of lactate dehydrogenase and pyruvate kinase in the DA-resistant B. gibsoni isolate were not different from those in the wild-type. These results indicated that the DA-resistant B. gibsoni isolate contained a higher ATP concentration than the wild-type, but the activity of the glycolysis pathway was not altered in the DA-resistant B. gibsoni isolate. However, we could not determine the mechanism of the high energy production of the DA-resistant B. gibsoni isolate. Further studies on the energy metabolism of B. gibsoni are necessary to clarify the mechanism of the high energy production in the DA-resistant B. gibsoni isolate.
机译：在我们之前的研究中，耐地米那嗪的乙酸双歧杆菌（G. gibsoni）分离株的寄生虫病水平持续低于野生型，表明该分离株中能量代谢的可能改变。因此，在本研究中，测量并比较了吉布森野生型和抗DA菌株中ATP，葡萄糖，乳酸和丙酮酸的浓度以及乳酸脱氢酶和丙酮酸激酶的活性，并比较了其含量。的能量产生和糖酵解途径的活性。结果，耐DA的长白僵菌分离株中的细胞内ATP和葡萄糖浓度显着高于野生型。同时，抗DA抗性的吉布森分离株中乳酸和丙酮酸的浓度以及乳酸脱氢酶和丙酮酸激酶的活性与野生型没有差异。这些结果表明，具有DA抗性的吉布森分离株比野生型具有更高的ATP浓度，但是在具有DA抗性的吉布森分离株中糖酵解途径的活性没有改变。但是，我们无法确定耐DA的长双歧杆菌的高能产生机理。为了阐明抗DA的长柄双歧杆菌菌株中高能量产生的机理，有必要对长柄双歧杆菌的能量代谢作进一步研究。

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