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首页> 外文期刊>The Japanese Journal of Veterinary Research >Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia.
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Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia.

机译：来自北海道和远东俄罗斯的tick传脑炎病毒的遗传和生物学比较。

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摘要

We compared the biological properties of Oshima 5-10 (tickborne encephalitis (TBE) virus isolated in Hokkaido, Japan) and Sofjin-HO (Far-Eastern subtype TBE virus) including plaque formation, virus replication and virus protein synthesis in BHK-21 cell cultures to reveal strain differences. We also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. Plaques of Oshima 5-10 were smaller than those of Sofjin-HO. Virus titres in culture fluid of Oshima 5-10 were 1/100 of those of Sofjin-HO at 9 and 12 h after infection. Less viral protein and RNA syntheses of strain Oshima 5-10 was observed than with Sofjin-HO. Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No differencebetween the 2 strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1.
机译：我们比较了大岛5-10（在日本北海道分离的ick传性脑炎（TBE）病毒）和Sofjin-HO（远东亚型TBE病毒）的生物学特性，包括BHK-21细胞中的噬斑形成，病毒复制和病毒蛋白合成揭示应变差异的文化。我们还确定了两种菌株的完整核苷酸序列，并比较了推导的氨基酸序列。大岛5-10的菌斑小于索非金河的菌斑。在感染后9和12小时，大岛5-10的培养液中的病毒滴度是Sofjin-HO的1/100。与Sofjin-HO相比，Oshima 5-10菌株的病毒蛋白和RNA合成较少。遗传分析显示1.4％的氨基酸与Sofjin-HO有差异。在病毒酶的基序序列，病毒蛋白的切割位点或NS1的糖基化位点上未检测到这两种菌株之间的差异。

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