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首页> 外文期刊>The Japanese Journal of Veterinary Research >Effects of cycloheximide treatment on in-vitro development of porcine parthenotes and somatic cell nuclear transfer embryos
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Effects of cycloheximide treatment on in-vitro development of porcine parthenotes and somatic cell nuclear transfer embryos

机译：环己酰亚胺处理对猪单性生殖和体细胞核移植胚胎体外发育的影响

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摘要

This study aimed to verify the beneficial effect of cycloheximide (CHX) treatment on the development of porcine somatic cell nuclear transfer (NT) embryos, which were constructed with enucleated oocytes and cumulus cells by using a single direct current (DC) pulse. In the first experiment, a single DC pulse applied to the induction of fusion and activation of NT embryos gave a high fusion rate. However, cleavage and subsequent development of fused couplets (NT embryos) to the blastocyst stage werepoor. Experiment 2 was conducted to determine whether CHX treatment could enhance metaphase II (MII) oocyte activation and improve the subsequent parthenogenetic development. After giving the DC pulse and incubation with or without CHX, M II oocytes incubated with CHX showed higher cleavage and development to blastocysts compared with those incubated without CHX (P < 0.05). Experiment 3 was carried out to verify the beneficial effect of CHX on the development of NT embryos. The NT embryos treated withthe DC pulse and CHX treatment showed higher cleavage and subsequent development compared with those treated with the DC pulse alone (P < 0.05). The present study demonstrates that CHX treatment enhances the electrical stimulus-induced activation of oocytes and NT embryos, and improves the subsequent development of parthenotes and NT embryos. The results indicate that protein synthesis inhibition treatment required for the induction of oocyte activation promotes the development of NT embryos.
机译：这项研究旨在验证环己酰亚胺（CHX）处理对猪体细胞核移植（NT）胚胎发育的有益作用，该胚胎由去核卵母细胞和卵丘细胞通过单个直流（DC）脉冲构建。在第一个实验中，单个DC脉冲应用于融合诱导和NT胚胎激活，具有很高的融合率。然而，卵裂和融合对联（NT胚胎）到胚泡期的发育较差。进行实验2以确定CHX处理是否可以增强中期II（MII）卵母细胞活化并改善随后的孤雌生殖发育。施加直流脉冲并与或不与CHX一起孵育后，与不与CHX孵育的卵母细胞相比，与CHX孵育的M II卵母细胞显示出更高的卵裂和发育为胚泡（P <0.05）。进行实验3以验证CHX对NT胚胎发育的有益作用。与仅用DC脉冲处理的NT胚胎相比，用DC脉冲和CHX处理的NT胚胎显示出更高的卵裂和随后的发育（P ＜0.05）。本研究表明，CHX处理增强了电刺激诱导的卵母细胞和NT胚胎的激活，并改善了单性生殖和NT胚胎的后续发育。结果表明诱导卵母细胞活化所需的蛋白质合成抑制处理促进NT胚的发育。

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