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首页> 外文期刊>The journal of clinical hypertension. >Effect of metabolic components on the incidence of cerebrovascular/cardiovascular disease among male workers in Japan: 3-year follow-up study
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Effect of metabolic components on the incidence of cerebrovascular/cardiovascular disease among male workers in Japan: 3-year follow-up study

机译：代谢成分对日本男性工人脑血管/心血管疾病发生率的影响：三年随访研究

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The induction of adequate vascularization, a major challenge in tissue engineering, has been tried with numerous methods but with unsatisfactory results. Adipose tissue, an active endocrine organ with dense vasculature, secretes a wide number of angiogenic and adipogenic factors and seems an attractive source for these bioactive factors. We produced a novel cell-free extract from mature human adipose tissue (adipose tissue extract [ATE]) and analyzed the ability of this extract to induce angiogenesis and adipogenesis in vitro and studied the cytokine and growth factor composition of ATE with ELISA and cytokine array. We demonstrate that ATE, when added as cell culture supplement, effectively induced triglyceride accumulation in human adipose stem cells at concentrations from 200 μg/mL upward in less than a week and caused elevated levels of adipocyte differentiation markers (proliferator-activated receptor gamma and acyl-CoA-binding protein) when treated with at least 350 μg/mL of ATE. ATE induced angiogenesis from 450 μg/mL upward after a week in vitro. ATE contained numerous angiogenic and adipogenic factors, for example, vascular endothelial growth factor, basic fibroblast growth factor, interleukin-6, adiponectin, angiogenin, leptin, and insulin-like growth factor-I, as well as lower levels of a wide variety of other cytokines. We here present a novel cell-free angiogenesis-and adipogenesis-inducing agent that is cell-free and easy to produce, and its effect is dose dependent and its composition can be easily modified. Therefore, ATE is a promising novel agent to be used for angiogenesis induction to overcome the challenge of vascularization and for adipogenesis induction in a wide variety of tissue engineering applications in vitro and in vivo. ATE is also efficient for reproduction and modeling of natural adipogenesis in vitro for, for example, obesity and diabetes studies.
机译：已经尝试了多种方法来诱导适当的血管化，这是组织工程学中的主要挑战，但是结果并不令人满意。脂肪组织是活跃的内分泌器官，具有密集的脉管系统，可分泌多种血管生成和脂肪形成因子，并且似乎是这些生物活性因子的诱人来源。我们从成熟的人体脂肪组织中制备了一种新型的无细胞提取物（脂肪组织提取物[ATE]），并分析了该提取物在体外诱导血管生成和脂肪生成的能力，并通过ELISA和细胞因子阵列研究了ATE的细胞因子和生长因子组成。我们证明，ATE作为细胞培养补充剂添加后，可以在不到一周的时间内从200μg/ mL的浓度有效诱导人脂肪干细胞中的甘油三酸酯蓄积，并引起脂肪细胞分化标志物（增殖物激活的受体γ和酰基）升高。 -CoA结合蛋白）用至少350μg/ mL ATE处理。体外培养一周后，ATE从450μg/ mL向上诱导血管生成。 ATE包含许多血管生成和脂肪生成因子，例如，血管内皮生长因子，碱性成纤维细胞生长因子，白介素-6，脂联素，血管生成素，瘦素和胰岛素样生长因子-I，以及较低水平的多种其他细胞因子。我们在这里提出了一种新型的无细胞血管生成和脂肪生成诱导剂，它是无细胞且易于生产的，其作用是剂量依赖性的，其组成可以很容易地改变。因此，ATE是一种有前途的新型试剂，可用于诱导血管生成，以克服血管生成的挑战，并用于体外和体内多种组织工程应用中的脂肪形成诱导。 ATE对于例如肥胖症和糖尿病研究的体外天然脂肪形成的复制和建模也是有效的。

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