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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >PHYSIOLOGY AND DEVELOPMENT OF PROTOPLASTS OBTAINED FROM FUCUS EMBRYOS USING LASER MICROSURGERY
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PHYSIOLOGY AND DEVELOPMENT OF PROTOPLASTS OBTAINED FROM FUCUS EMBRYOS USING LASER MICROSURGERY

机译：激光显微术从洋槐胚中获得原生质体的生理学和发育

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摘要

Protoplasts from rhizoids of two-celled Fucus embryos were obtained using laser microsurgery. They were characterised by a membrane potential similar to that of a rhizoid cell in situ and a rapid regeneration of the cell wall. Nucleated subprotoplasts with regenerated cell wall were capable of photopolarisation as zygotes. These pseudozygote developed into miniature embryos similar to zygotic embryos. The nucleus appeared to be necessary for pseudozygote polarisation but the transcription inhibitor, actinomycin D, though preventing zygote polarisation, was inactive on pseudozygotes. A role for the nucleus as a geometric reference is discussed. [References: 59]
机译：使用激光显微手术从两细胞悬钩子胚胎的根状体获得原生质体。它们的特征在于其膜电位类似于原状根状细胞的膜电位以及细胞壁的快速再生。具有再生细胞壁的有核亚原生质体能够作为受精卵进行光极化。这些假合子发育成类似于合子胚的微型胚胎。核似乎是假合子极化所必需的，但是转录抑制剂放线菌素D虽然可以防止合子极化，但对假合子没有活性。讨论了原子核作为几何参考的作用。 [参考：59]

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