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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >MALE GAMETOPHYTE DEVELOPMENT IN PLUMBAGO ZEYLANICA - CYTOPLASM LOCALIZATION AND CELL DETERMINATION IN THE EARLY GENERATIVE CELL
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MALE GAMETOPHYTE DEVELOPMENT IN PLUMBAGO ZEYLANICA - CYTOPLASM LOCALIZATION AND CELL DETERMINATION IN THE EARLY GENERATIVE CELL

机译：墨西哥产雄性胎生藻发育-早期生殖细胞中细胞质的定位和细胞的确定。

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The behavior of the generative cell during male gametophyte development in Plumbago zeylanica was examined by epifluorescence microscopy and electron microscopy with organelle nucleoid as a cytoplasm marker. When the thin sections stained with 4',6-diamidino-2-phenylindole (DAPI) were observed under an epifluorescence microscope, two types of fluorescence spots were detected in the cytoplasm of the pollen cells before the second mitosis. The spots emitting stronger fluorescence were confirmed as plastid nucleoids and those emitting dimmer fluorescence were mitochondrial nucleoids. Before the first mitosis, both plastid and mitochondrial nucleoids distributed randomly in the cytoplasm of the microspore. A small lenticular generative cell formed with attachment to the interior of the intine after the mitosis. Small vacuoles were found in the lenticular cell. In the cytoplasm of the lenticular cell, both plastid nucleoids and the small vacuoles were distributed randomly at the very beginning but began to migrate in opposite directions immediately. Plastid nucleoids aggregated to the side of the cell that faces the pollen center and the small vacuoles aggregated to the side of the cell that attaches to the intine. As the result, the lenticular generative cell appeared highly polarized in cytoplasm location soon after the first mitosis. In accordance with the definition of the cytoplasm polarization, the primary wall between the generative and the vegetative cells began to flex and the lenticular generative cell started to protrude towards the pollen center. When the generative cell peeled away from the intine, it was spherical in shape with the pole that aggregated plastids towards the vegetative nucleus. But the cell direction appeared to be transformed immediately. The pole that aggregated small vacuoles turned to the position towards the vegetative nucleus and the pole that aggregated plastid nucleoids turned to the position countering to the vegetative nucleus. A cellular protuberance formed at the edge of the pole that aggregated small vacuoles and elongated into a tapered end that got into contact with the vegetative nucleus. The polarization of the cytoplasm kept constant throughout the second mitosis. The small vacuoles that apportioned to the sperm cell which attached the vegetative nucleus (the leading sperm cell) disappeared during sperm cell maturation. Plastid nucleoids were apportioned to the other sperm cell (the trailing sperm cell) completely. Mitochondrial nucleoids became undetectable after the second mitosis. [References: 14]
机译：通过表观荧光显微镜和电子显微镜以细胞器核苷酸作为细胞质标记，检查了玉米中雄配子体发育过程中生殖细胞的行为。当在落射荧光显微镜下观察到被4'，6-二ino基-2-苯基吲哚（DAPI）染色的薄片时，在第二次有丝分裂之前，花粉细胞的细胞质中检测到两种类型的荧光斑点。发出较强荧光的斑点被确认为质体核苷，发出较暗荧光的斑点被确认为线粒体核苷。在第一次有丝分裂之前，质体和线粒体的核苷都随机分布在小孢子的细胞质中。小有突状突状生成细胞，在有丝分裂后附着于大肠内部。在透镜状细胞中发现小液泡。在柱状细胞的细胞质中，质体核苷和小液泡一开始都是随机分布的，但立即开始向相反的方向迁移。质子核苷聚集在细胞面向花粉中心的一侧，而小液泡聚集在细胞附着于花粉的一侧。结果，在第一次有丝分裂后不久，双凸状生殖细胞在细胞质位置出现高度极化。根据细胞质极化的定义，生殖细胞和营养细胞之间的原壁开始弯曲，而双凸状生殖细胞开始向花粉中心突出。当生殖细胞从大肠上剥下时，它是球形的，带有向质体核聚集的质体的极。但是细胞方向似乎立即转变了。聚集小液泡的极点朝着营养核的位置，聚集质体核苷的极点朝着与营养核相反的位置。在杆的边缘形成的细胞突起聚集小液泡，并延伸到与营养核接触的锥形末端。在第二次有丝分裂期间，细胞质的极化保持恒定。在精子细胞成熟过程中，分配给附着有营养核（精子细胞）的精子细胞的小液泡消失了。质体核苷完全分配给另一个精子细胞（尾部精子细胞）。第二次有丝分裂后，线粒体的核苷变得不可检测。 [参考：14]

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