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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >In vivo pollen tube cell of Arabidopsis thaliana I. Tube cell cytoplasm and wall
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In vivo pollen tube cell of Arabidopsis thaliana I. Tube cell cytoplasm and wall

机译：拟南芥的体内花粉管细胞I.管细胞的细胞质和壁

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摘要

Pollen tubes are an excellent system for the study of polarized tip growth, cell movement, and cell-to-cell communication in plants. We report the first description at the transmission electron microscope level of cryofixed, in vivo grown pollen tubes. The tube cell of Arabidopsis thaliana growing in the style can be divided into four zones, similar to those reported for pollen tube cells of members of the family Solanaceae. Some distinct differences from data reported for other species include the morphology of the clear zone at the tip, the composition of the tip wall, and the apparent lack of arabinogalactan proteins in the pollen tube and surrounding transmitting tissue. The clear zone of A. thaliana is packed with vesicles which do not form the inverted cone found in other species and that fuse in clusters with the plasmalemma in the tip region. Immunocytochemical analyses revealed that the pollen tube has a primary, pectic wall composed solely of highly esterified homogalacturonans at the tip. This wall becomes increasingly thicker basipetally and is composed of both highly esterified and lowly esterified homogalacturonans behind the clear zone. A secondary callosic wall appears behind the clear zone and becomes thicker basipetally. Using a variety of probes, we have been unable to identify any arabinogalactan proteins in the pollen tube cell or in the transmitting tissue of the style. Unlike our earlier reports of the extracellular matrices of the septum transmitting tissue of the ovary, the stylar matrices were found to be rich in both highly and lowly esterified homogalacturonans. [References: 77]
机译：花粉管是研究极化尖端生长，细胞运动和植物细胞间通讯的极佳系统。我们在冷冻固定的，体内生长的花粉管的透射电子显微镜水平上报告了第一个描述。以这种方式生长的拟南芥的管细胞可分为四个区域，类似于茄科成员的花粉管细胞的报道。与报道的其他物种的数据有一些明显的差异，包括尖端透明区域的形态，尖端壁的组成以及花粉管和周围传播组织中明显缺乏阿拉伯半乳聚糖蛋白。拟南芥的透明区充斥着囊泡，囊泡不形成其他物种中发现的倒锥，并与尖端区域的浆膜成簇融合。免疫细胞化学分析表明，花粉管的顶端有一个主果胶壁，仅由高度酯化的同半乳糖醛酸聚糖组成。该壁在基底上越来越厚，由在透明区后面的高度酯化和低酯化的同半乳糖醛酸组成。次生愈伤组织壁出现在透明区的后面，并在基底上变厚。使用多种探针，我们无法鉴定花粉管细胞或该类型的传播组织中的任何阿拉伯半乳聚糖蛋白。与我们先前关于卵巢中隔传递组织的细胞外基质的报道不同，发现stylar基质富含高度和低酯化的同型半乳糖醛酸。 [参考：77]

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