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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Rapid detection of plasmodesmata in purified cell walls
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Rapid detection of plasmodesmata in purified cell walls

机译：快速检测纯化细胞壁中的浆膜

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摘要

Plasmodesmata are complex channels within the plant cell wall, which create plasma membrane and symplastic continuity between neighbouring cells. To detect plasmodesmata in cell wall preparations from Nicotiana de elandii, we have used 3,3'-dihexyloxacarbocyanine iodide (DiOC(6)), a cationic amphiphilic fluorescent probe, widely employed for general studies of membrane structure and dynamics. Punctate fluorescent staining was readily seen in pit fields, small depressions within the cell wall known to be rich in plasmodesmata. Scanning electron microscopy was used to demonstrate that the punctate staining corresponded to plasmodesmata. Treatment of cell wail fragments with chloroform-methanol to remove lipids did not alter the staining of plasmodesmata. In contrast, pronase E-sodium dodecyl sulfate treatment completely abolished staining, indicating that the DiOC(6) labelling of plasmodesmata may be protein rather than lipid specific. Although not membrane mediated, DiOC(6) staining of plasmodesmata is a simple, rapid, and specific tool for the detection of plasmodesmata in isolated cell walls and will prove useful for studies of plasmodesmal location, structure, and composition. [References: 22]
机译：浆线虫是植物细胞壁内的复杂通道，其在邻近细胞之间产生质膜和共生连续性。为了检测来自尼古拉斯烟草的细胞壁制剂中的胞浆菌，我们使用了阳离子两亲性荧光探针3,3'-二己基氧杂碳菁碘化物（DiOC（6）），广泛用于膜结构和动力学的一般研究。在凹坑中很容易看到点状荧光染色，这是细胞壁内的小凹陷，已知富含胞质。扫描电子显微镜用于证明点状染色对应于胞浆菌。用氯仿-甲醇处理细胞壁碎片以去除脂质，并没有改变质粒的染色。相反，链酶E-十二烷基硫酸钠处理完全废除了染色，表明纤毛虫的DiOC（6）标记可能是蛋白质而不是脂质特异性的。尽管不是膜介导的，但等离子的DiOC（6）染色是检测孤立细胞壁中的等离子的简单，快速且特定的工具，将证明对等离子的位置，结构和组成的研究很有用。 [参考：22]

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