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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Dynamic rearrangements of transvacuolar strands in BY-2 cells imply a role of myosin in remodeling the plant actin cytoskeleton
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Dynamic rearrangements of transvacuolar strands in BY-2 cells imply a role of myosin in remodeling the plant actin cytoskeleton

机译：BY-2细胞中跨血管链的动态重排暗示了肌球蛋白在重塑植物肌动蛋白细胞骨架中的作用

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摘要

Plant cells typically contain a large central vacuole that confines the cytoplasm and organelles to the periphery of the cell and the vicinity of the nucleus. These two domains are often connected by transvacuolar strands (TVS), thin tubular structures that traverse the vacuole. The TVS are thought to act as important transport routes for the distribution of organelles and metabolites, and also to play a role in the positioning of the nucleus. Most TVS depend on internal actin filaments for their existence, and rearrangements of TVS can therefore indicate modifications in the actin cytoskeleton. In this study we describe time-lapse observations of tobacco BY-2 suspension-cultured cells that document the dynamic behavior of TVS. The TVS formed, branched, and collapsed, and their attachment points in the nuclear or cortical cytoplasm, as well as on other TVS, moved around. These dynamic rearrangements were inhibited within 5 min by the myosin inhibitor 2,3-butanedione monoxime (BDM). In particular, the movements of TVS attachment points and the variations in TVS length were significantly reduced in the presence of the drug. Similarly, movements of the nucleus were reduced by two thirds in BDM-treated cells. The number of TVS, together with the number of attachment and branch points, also dropped during BDM treatment. All effects of BDM on TVS dynamics were reversible upon removal of the drug. These results suggest a role for myosin motors in the rearrangement of TVS, which is likely to occur through their interaction with actin filaments.
机译：植物细胞通常包含一个较大的中央液泡，该液泡将细胞质和细胞器限制在细胞外围和细胞核附近。这两个结构域通常通过横空泡的链（TVS）连接，这是穿过液泡的细管状结构。 TVS被认为是细胞器和代谢物分布的重要运输途径，并在细胞核的定位中发挥作用。大多数TVS的存在依赖于内部肌动蛋白丝，因此TVS的重排可以表明肌动蛋白细胞骨架的修饰。在这项研究中，我们描述了记录烟草TVS动态行为的烟草BY-2悬浮培养细胞的延时观察。 TVS形成，分支和折叠，它们在核或皮层细胞质以及其他TVS中的附着点四处移动。这些动态重排在5分钟内被肌球蛋白抑制剂2,3-丁二酮单肟（BDM）抑制。特别地，在存在药物的情况下，TVS附着点的移动和TVS长度的变化明显减少。同样，在BDM处理的细胞中，细胞核的运动减少了三分之二。在BDM治疗期间，TVS的数量以及附着点和分支点的数量也减少了。去除药物后，BDM对TVS动力学的所有影响都是可逆的。这些结果暗示了肌球蛋白马达在TVS的重排中的作用，这很可能通过它们与肌动蛋白丝的相互作用而发生。

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