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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Mitochondrial DNA decreases during pollen development in rapeseed (Brassica napus L.), but mitochondrial linear-plasmid-encoded RNA polymerase persists in mature pollen
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Mitochondrial DNA decreases during pollen development in rapeseed (Brassica napus L.), but mitochondrial linear-plasmid-encoded RNA polymerase persists in mature pollen

机译：油菜（花椰菜）花粉发育过程中线粒体DNA减少，但线粒体线性质粒编码的RNA聚合酶在成熟花粉中持续存在

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摘要

Mitochondrial DNA in the male reproductive cells of rapeseed (Brassica napus L.) was monitored by fluorescence microscopy of Technovit 7100 resin sections double-stained with 4',6-diamidino-2-phenylindole and 3,3'-dihexyloxacarbocyanine iodide. Mitochondrial DNA progressively decreased during pollen development and disappeared in mature pollen. This result corresponds well with the maternal inheritance of mitochondria in rapeseed determined by previous genetic analyses. To better characterize the mode of inheritance of the mitochondrial linear plasmid in rapeseed, which is transmitted through pollen, we analyzed by indirect immunofluorescence microscopy the expression and localization of ORF6 protein, a putative RNA polymerase encoded by the plasmid. ORF6 protein was expressed in mature pollen and specifically localized in the cytoplasm of sperm cells in the mature pollen. This suggests that the genes encoded by the plasmid DNA are transcribed in the mature pollen by its own RNA polymerase (ORF6 protein) and that the gene expression in the generative cells may be needed for transmission of plasmid DNA through the pollen.
机译：油菜（甘蓝型油菜）雄性生殖细胞中的线粒体DNA通过用4'，6-二mid基-2-苯基吲哚和3,3'-二己基氧杂碳菁碘化物双染色的Technovit 7100树脂切片的荧光显微镜进行监测。线粒体DNA在花粉发育过程中逐渐减少，而在成熟花粉中消失。该结果与通过先前的遗传分析确定的油菜籽中线粒体的母体遗传非常吻合。为了更好地表征油菜中线粒体线性质粒通过花粉传播的遗传模式，我们通过间接免疫荧光显微镜分析了ORF6蛋白的表达和定位，ORF6蛋白是一种由质粒编码的假定的RNA聚合酶。 ORF6蛋白在成熟花粉中表达，并特异性地定位在成熟花粉中的精子细胞质中。这表明由质粒DNA编码的基因通过其自身的RNA聚合酶（ORF6蛋白）在成熟的花粉中转录，并且可能需要在生殖细胞中的基因表达才能使质粒DNA通过花粉传播。

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