• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cytochemistry and immunolocalisation of polysaccharides and proteoglycans in the endosperm of green Arabica coffee beans
【24h】

Cytochemistry and immunolocalisation of polysaccharides and proteoglycans in the endosperm of green Arabica coffee beans

机译：绿色阿拉比卡咖啡豆胚乳中多糖和蛋白聚糖的细胞化学和免疫定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The major noncellulosic polysaccharides and proteoglycans in the coffee bean (Coffea arabica) cell wall are (galacto)mannans and arabinogalactan proteins. Immunological and chemical probes demonstrated that the mannans and arabinogalactan proteins were located continuously across the width of the cell wall, but that the concentration of different structural epitopes within these polysaccharide types showed considerable spatial variation. For the mannans this was implied by the striated pattern demonstrated by fluctuation of the affinity between the mannan monoclonal antibody BGM C6 and (galacto)mannan. The arabinogalactan proteins labelled by the Yariv reagent and the arabinogalactan protein-specific antibody LM2 appeared to be located in all regions of the wall except the middle lamella. but showed some differences in intensity of labelling. However, the LM6 antibody, specific for (1-->5)alpha-arabinan epitopes, was located only as a compact region adjacent to the cell lumen in the body of the endosperm; though, it did label throughout the wall of epidermal cells. This implied that either some of the more highly arabinosylated arabinogalactan proteins contained contiguous 5-arabinosyl residues or that a rhamnogalacturonan which contained 5-arabinosyl residues as side chains existed in the cell wall. In either case the polymers were very restricted in their distribution. A second category of pectin, a homogalacturonan detected by JIM7, was located only in the middle lamella region. The architecture of the wall, as revealed by resin etching, appeared to reflect the chemical heterogeneity, with three distinct physical zones identifiable in a cross section across a single wall.
机译：咖啡豆（阿拉伯咖啡）细胞壁中主要的非纤维素多糖和蛋白聚糖是（半乳）甘露聚糖和阿拉伯半乳聚糖蛋白。免疫学和化学探针表明甘露聚糖和阿拉伯半乳聚糖蛋白连续分布在细胞壁的整个宽度上，但是这些多糖类型中不同结构表位的浓度显示出相当大的空间变化。对于甘露聚糖，这是由甘露聚糖单克隆抗体BGM C6和（半乳）甘露聚糖之间的亲和力波动证明的横纹图案暗示的。 Yariv试剂标记的阿拉伯半乳聚糖蛋白和阿拉伯半乳聚糖蛋白特异性抗体LM2似乎位于除中层以外的所有壁区域中。但在标记强度上显示出一些差异。但是，特异于（1-> 5）α-阿拉伯糖基抗原决定簇的LM6抗体仅位于紧靠胚乳体内细胞管腔的紧密区域。但是，它确实标记了整个表皮细胞壁。这暗示某些更高阿拉伯糖基化的阿拉伯半乳聚糖蛋白包含连续的5-阿拉伯糖基残基，或者在细胞壁中存在包含作为侧链的5-阿拉伯糖基残基的鼠李糖半乳糖醛酸聚糖。无论哪种情况，聚合物的分布都受到很大限制。果胶的第二类，即由JIM7检测到的同型半乳糖醛酸，仅位于中片区。树脂蚀刻显示的壁结构似乎反映了化学异质性，在单个壁的横截面中可识别出三个不同的物理区域。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号