Testing for endocytosis in plants

Aniento F; Robinson DG

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Testing for endocytosis in plants

机译：测试植物内吞作用

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For many years endocytosis has been regarded with great scepsis by plant physiologists. Although now generally accepted, care must still be taken with experiments designed to demonstrate endocytic uptake at the plasma membrane. We have taken a critical look at the various agents which are in use as markers for plant endocytosis, pointing out pitfalls and precautions which should be taken. We also take this opportunity to introduce the tyrphostins - tyrosine kinase inhibitors -, which also seem to prevent endocytosis in plants.

机译：多年以来，植物生理学家一直认为内吞作用会引起极大的惊吓。尽管现在已被普遍接受，但仍必须谨慎进行旨在证明质膜吞噬内吞的实验。我们仔细研究了用作植物内吞标记的各种试剂，指出了陷阱和应采取的预防措施。我们还借此机会介绍了酪氨酸激酶抑制剂（酪氨酸激酶抑制剂），它也可以阻止植物的内吞作用。

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《Protoplasma: An International Journal of Cell Biology》 |2005年第2期|共9页
作者
Aniento F; Robinson DG;
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正文语种 eng
中图分类细胞形态学;
关键词
biotinylated marker; FM lipophilic styryl dye; Lucifer Yellow; tyrphostin; wortmannin; FLUID-PHASE ENDOCYTOSIS; LUCIFER YELLOW CH; RECEPTOR-MEDIATED ENDOCYTOSIS; CULTURED SOYBEAN CELLS; AP-2 ADAPTER COMPLEX; MEDIUM-CHAIN SUBUNIT; GNOM ARF-GEF; PLASMA-MEMBRANE; TRANSFERRIN RECEPTOR; FLUORESCENT DYES;

机译：生物素标记物;FM亲脂性苯乙烯基染料;路西法黄;酪氨酸磷酸酶;渥曼青霉素;流体相的内吞作用;卢西尔黄CH;受体介导的内吞作用;培养的大豆细胞;AP-2适配器复合物;中链亚基;GNOM-ARF -膜;转铁蛋白受体;荧光染料;

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1. Testing for endocytosis in plants [J] . F. Aniento, D. G. Robinson Protoplasma . 2005,第1a2期

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5. The lactoferrin receptor mediates lactoferrin endocytosis and is involved in the effects of lactoferrin on intestinal proliferation and characterization of the human lactoferrin receptor gene promoter. [D] . Jiang, Rulan. 2010

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6. Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants [O] . Madhumitha Narasimhan, Alexander Johnson, Roshan Prizak, 2020

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7. Endocytosis in Plant Protoplasts: Visualization and Quantitation of Fluid-Phase Endocytosis Using Silver-Enhanced Bovine Serum Albumin-Gold Abstract [O] . Villanueva Marco A., Taylor Josephine, Griffing Lawrence R. 1993

机译：植物原生质体中的内吞作用：使用银增强的牛血清白蛋白-金对液相内吞作用的可视化和定量分析摘要

Testing for endocytosis in plants

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