• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Verticillium dahliae toxin induced alterations of cytoskeletons and nucleoli in Arabidopsis thaliana suspension cells
【24h】

Verticillium dahliae toxin induced alterations of cytoskeletons and nucleoli in Arabidopsis thaliana suspension cells

机译：黄萎病毒素诱导拟南芥悬浮细胞细胞骨架和核仁的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In plant cells, cytoskeletons play important roles in response to biotic and abiotic stresses. However, little is known about the dynamics of cytoskeletons when cells are attacked by unphysical stress factors such as elicitors and toxins. We report here that the toxin of Verticillium dahliae (VD toxin) induced changes of microfilaments (MFs) and microtubules (MTs) in Arabidopsis thaliana suspension-cultured cells. When cells were treated with a low concentration of VD toxin, MFs were disrupted ordinally from the cortex to the perinuclear region, and then recovered spontaneously; but the MTs persisted. The MFs in the perinuclear region showed more resistance to VD toxin than the cortical ones. In contrast, when cells were treated with a high concentration of VD toxin, MFs and MTs were disrupted sooner and more severely and did not recover spontaneously. Treatments with high concentrations of VD toxin also induced changes of nucleoli. At the early stages of treatment, a nucleus had a single ring-shaped nucleolus. At the later stages, multiple smaller and more brightly fluorescing nucleoli emerged in a single nucleus. Disrupted MFs could be recovered by removing the VD toxin before the ringshaped nucleoli appeared. All these results showed that MFs and MTs play important roles in the early defense responses against VD toxin in Arabidopsis suspension cells. The cytoskeletons may be used as sensors and effectors monitoring the defense reactions. The changes of nucleoli induced by VD toxin should be important characteristics of cell death.
机译：在植物细胞中，细胞骨架在应对生物和非生物胁迫中起重要作用。但是，当细胞受到非物理应激因素（如引发剂和毒素）攻击时，细胞骨架的动力学知之甚少。我们在这里报告，黄萎病菌的毒素（VD毒素）诱导拟南芥悬浮培养细胞中的微丝（MF）和微管（MT）的变化。当用低浓度的VD毒素处理细胞时，MF通常从皮层到核周区域被破坏，然后自发地恢复。但是MT坚持了下来。核周区域的MF对皮质类固醇的抵抗力比皮质高。相反，当用高浓度的VD毒素处理细胞时，MF和MT会被更快，更严重地破坏，并且无法自发恢复。高浓度VD毒素的治疗也诱导了核仁的改变。在治疗的早期，核具有单个环形核仁。在随后的阶段，单个核中出现了多个更小，更明亮的荧光核仁。可以通过在环状核仁出现之前去除VD毒素来恢复破坏的MF。所有这些结果表明，MF和MT在拟南芥悬浮细胞中对VD毒素的早期防御反应中起重要作用。细胞骨架可用作监测防御反应的传感器和效应子。 VD毒素诱导的核仁变化应该是细胞死亡的重要特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号