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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Immunodetection of pectin and arabinogalactan protein epitopes during pollen exine formation of Beta vulgaris L.
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Immunodetection of pectin and arabinogalactan protein epitopes during pollen exine formation of Beta vulgaris L.

机译：β寻常型花粉外壁形成过程中果胶和阿拉伯半乳聚糖蛋白表位的免疫检测。

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摘要

We present the results of ultrastructural and immunocytochemical studies of sugar beet microsporocytes during the developmental phase that begins with the first meiotic metaphase and ends with the formation of young tetrads. The most prominent feature noted during this period of microsporogenesis was the presence of numerous cisternae of endoplasmic reticulum which frequently lie perpendicular to the surface of the plasma membrane and eventually fuse to it. Microscopic observations have been combined with the detection of several carbohydrate epitopes representing pectins and arabinogalactan proteins in the primexine and incipient exine. Pectin domains that possess both low and highly methylesterified epitopes, as well as pectin side chains enriched in (1 -> 4)-beta-D-galactose residues, are deposited in this young microspore wall. The epitopes of arabinogalactan protein that bind to JIM13, JIM8, and LM2 antibodies are localised within the callose wall surrounding posttelophase tetrads. The possibility of endoplasmic-reticulum involvement in the synthesis, transport, or metabolism of several microspore wall compounds is discussed.
机译：我们介绍了甜菜微孢子细胞在发育阶段的超微结构和免疫细胞化学研究的结果，该阶段从第一个减数分裂中期开始，到年轻的四分体的形成结束。在微孢子发生期间，最显着的特征是存在大量内质网的池，这些池经常垂直于质膜的表面并最终融合到质膜的表面。显微镜观察已与检测primemine和excipient exine中代表果胶和阿拉伯半乳聚糖蛋白的几种碳水化合物表位相结合。具有低和高度甲基酯化的表位，以及富含（1-> 4）-β-D-半乳糖残基的果胶侧链的果胶结构域沉积在这个年轻的小孢子壁中。与JIM13，JIM8和LM2抗体结合的阿拉伯半乳聚糖蛋白的表位位于末期四联体的call壁中。讨论了内质网参与几种小孢子壁化合物的合成，转运或代谢的可能性。

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