首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkorper in Aspergillus nidulans
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Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkorper in Aspergillus nidulans

机译:钙调蛋白集中在菌丝生长的顶点,并定位于构巢曲霉中的Spitzenkorper

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摘要

The calmodulin (CaM) localization pattern in the growing hyphal tip of Aspergillus nidulans was studied with the functional GFP:: CaM fusion protein. A faint tip-high gradient of CaM was found in the growing hyphal tip, with CaM highly localized in the region corresponding to the Spitzenkorper forming a bright granule. The position of highly concentrated CaM in the extreme apex seemed to determine the orientation of the hypha. The normal pattern of CaM localization was also shown to be dependent on the integrated actin cytoskeleton. When the growth of the hyphal tip ceased, CaM failed to localize in the bright granule and was evenly distributed in the hyphal tip. These findings suggest that CaM may play an important role in establishing and maintaining apical organization, morphogenesis, and growth in Aspergillus nidulans.
机译:用功能性GFP :: CaM融合蛋白研究了构巢曲霉菌丝生长的钙调蛋白(CaM)定位模式。在生长的菌丝尖端发现微弱的CaM尖端高梯度,CaM高度定位在与Spitzenkorper对应的区域中,形成明亮的颗粒。高浓度CaM在极尖的位置似乎决定了菌丝的方向。 CaM定位的正常模式也显示取决于整合的肌动蛋白细胞骨架。当菌丝尖端的生长停止时,CaM不能定位在明亮的颗粒中,而是均匀分布在菌丝尖端中。这些发现表明,CaM可能在构巢曲霉的根尖组织,形态发生和生长中起重要作用。

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