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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >LIGHT AND ELECTRON MICROSCOPIC STUDY OF THE BACTERIAL ADHESION TO TERMITE FLAGELLATES APPLYING LECTIN CYTOCHEMISTRY
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LIGHT AND ELECTRON MICROSCOPIC STUDY OF THE BACTERIAL ADHESION TO TERMITE FLAGELLATES APPLYING LECTIN CYTOCHEMISTRY

机译：白细胞介素对白蚁的细菌粘附的光电子显微镜研究

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Many of the flagellates inhabiting the hindgut of lower termites are associated with ectobiotic, rod-like bacteria or spirochetes. Different types of attachment sites are present. Electron dense material underlies, e.g., the plasma membrane of Joenia annectens at the contact site, whereas other attachment sites do not show any visible specializations. The host cell's glycocalyx may, however, be reduced at the attachment sites as it is the case in Devescovina glabra. The thick glycocalyx of Stephanonympha nelumbium is not changed at the sites where bacterial rods attach, but spirochetes penetrate to a certain extent. Bacteria which colonize the extracellular surface structures of Microrhopalodina multinucleata express their own glycocalyx to mediate a contact. In this study we focussed on the examination of one common mode of interaction between bacteria and their host cells, i.e., adhesion via lectins and sugars. The sugar composition was analysed by light and electron microscopic labelling experiments using the lectins Con A, WGA and SBA. In general, only the posterior body surface of Joenia which is colonized with bacteria is labelled. The demonstrated sugars are found in fibrous glycocalyx portions surrounding the attachment sites of the bacteria. Such glycocalyx fibres in combination with the electron dense material supporting the attachment sites seem to be the prerequisites for bacterial attachment. In D. glabra, however, a role for sugars in mediating the attachment could not be demonstrated. Removal of the ectobiotes using antibiotics revealed that the specialized contact sites of Joenia are present in the absence of bacteria and thus possibly serve to attract bacteria. Nothing, however, remains of the former attachment sites in bacteria-free Devescovina cells. Attachment sites in this case could be induced by bacterial contact. There is not one general mechanism for bacterial attachment to termite flagellates; rather, adhesion seems to follow different strategies. [References: 43]
机译：居住在下白蚁后肠的许多鞭毛虫与外生，杆状细菌或螺旋体有关。存在不同类型的附着点。电子致密材料位于（例如）乔尼亚（Joenia）质膜在接触部位处，而其他附着部位则未显示任何可见的专长。但是，宿主细胞的糖萼可能会在附着位点被还原，就像在欧洲黑背果蝇中那样。 Stephanonympha nelumbium的厚糖萼在细菌杆附着的位置没有改变，但是螺旋体在一定程度上穿透了。定居于多核微核孢菌的细胞外表面结构的细菌表达它们自己的糖萼以介导接触。在这项研究中，我们集中于检查细菌与其宿主细胞之间一种常见的相互作用模式，即通过凝集素和糖的粘附。使用凝集素Con A，WGA和SBA通过光和电子显微镜标记实验分析糖的组成。通常，仅标记被细菌定殖的乔尼亚氏菌的后体表面。在细菌附着部位周围的纤维糖萼部分中发现了证实的糖。这种糖萼纤维与支持附着位点的电子致密材料的结合似乎是细菌附着的先决条件。然而，在D. glabra中，糖在介导附着中的作用无法得到证实。使用抗生素去除外生菌后发现，乔尼娅的专门接触部位不存在细菌，因此可能会吸引细菌。但是，在无细菌的蛇毒属植物中，以前的附着位点仍然没有。在这种情况下，附着位点可以由细菌接触诱导。没有一种细菌附着在白蚁鞭毛上的一般机制。相反，粘附似乎遵循不同的策略。 [参考：43]

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