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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The effect of bupivacaine center dot HCl on the physical properties of neuronal membranes
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The effect of bupivacaine center dot HCl on the physical properties of neuronal membranes

机译：布比卡因中心点HCl对神经元膜物理特性的影响

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摘要

Fluorescent probe techniques were used to evaluate the effect of bupivacaine center dot HCl on the physical properties (transbilayer asymmetric lateral and rotational mobilities, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. An experimental procedure was used based on selective quenching of both 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer (RET) from the tryptophans of membrane proteins to Py-3-Py. Bupivacaine center dot HCl increased the bulk lateral and rotational mobilities, and annular lipid fluidity in SPMVs lipid bilayers, and had a greater fluidizing effect on the inner monolayer than that of the outer monolayer. The magnitude of increasing effect on annular lipid fluidity in SPMVs lipid bilayer induced by bupivacaine center dot HCl was significantly far greater than magnitude of increasing effect of the drug on the lateral and rotational mobilities of bulk SPMVs lipid bilayer. It also caused membrane proteins to cluster. These effects of bupivacaine center dot HCl on neuronal membranes may be responsible for some, though not all, of the local anesthetic actions of bupivacaine center dot HCl.
机译：荧光探针技术用于评估布比卡因中心点HCl对从牛大脑皮层分离的突触体质膜囊泡（SPMV）的物理性质（跨双层不对称的横向和旋转运动，环形脂质流动性和蛋白质分布）的影响。基于三硝基苯基对1，3-二（1-吡啶基）丙烷（Py-3-Py）和1,6-二苯基-1,3,5-己三烯（DPH）的选择性淬灭，采用了一种实验程序，以及从膜蛋白色氨酸到Py-3-Py的无辐射能量转移（RET）。布比卡因中心点HCl增加了SPMVs脂质双层中的整体横向和旋转运动，以及环状脂质流动性，并且对内部单层的流化作用大于外部单层的流化作用。布比卡因中心点HCl对SPMVs脂质双层中环状脂质流动性增加作用的增加幅度远远大于药物对SPMVs脂质双层整体横向和旋转运动的增加作用的作用程度。它还引起膜蛋白聚集。布比卡因中心点HCl对神经元膜的这些作用可能是布比卡因中心点HCl的部分（但不是全部）局部麻醉作用的原因。

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