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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Detection and subcellular localization of dehydrin-like proteins in quinoa (Chenopodium quinoa Willd.) embryos
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Detection and subcellular localization of dehydrin-like proteins in quinoa (Chenopodium quinoa Willd.) embryos

机译：奎奴亚藜（Chenopodium quinoa Willd。）胚胎中脱水素样蛋白的检测和亚细胞定位

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The aim of this study was to characterize the dehydrin content in mature embryos of two quinoa cultivars, Sajama and Baer La Union. Cultivar Sajama grows at 3600-4000 m altitude and is adapted to the very arid conditions characteristic of the salty soils of the Bolivian Altiplano, with less than 250 mm of annual rain and a minimum temperature of -1 degrees C. Cultivar Baer La Union grows at sea-level regions of central Chile and is adapted to more humid conditions (800 to 1500 mm of annual rain), fertile soils, and temperatures above 5 degrees C. Western blot analysis of embryo tissues from plants growing under controlled greenhouse conditions clearly revealed the presence of several dehydrin bands (at molecular masses of approximately 30, 32, 50, and 55 kDa), which were common to both cultivars, although the amount of the 30 and 32 kDa bands differed. Nevertheless, when grains originated from their respective natural environments, three extra bands (at molecular masses of approximately 34, 38, and 40 kDa), which were hardly visible in Sajama, and another weak band (at a molecular mass of approximately 28 kDa) were evident in Baer La Union. In situ immunolocalization microscopy detected dehydrin-like proteins in all axis and cotyledon tissues. At the subcellular level, dehydrins were detected in the plasma membrane, cytoplasm and nucleus. In the cytoplasm, dehydrins were found associated with mitochondria, rough endoplasmic reticulum cisternae, and proplastid membranes. The presence of dehydrins was also recognized in the matrix of protein bodies. In the nucleus, dehydrins were associated with the euchromatin. Upon examining dehydrin composition and subcellular localization in two quinoa cultivars belonging to highly contrasting environments, we conclude that most dehydrins detected here were constitutive components of the quinoa seed developmental program, but some of them (specially the 34, 38, and 40 kDa bands) may reflect quantitative molecular differences associated with the adaptation of both cultivars to contrasting environmental conditions.
机译：这项研究的目的是表征两个藜麦品种Sajama和Baer La Union的成熟胚中的脱水素含量。萨哈玛栽培种生长在3600-4000 m的高度，并且适应玻利维亚高原的咸土的非常干旱的条件，年降雨量少于250毫米，最低温度为-1摄氏度。在智利中部的海平面区域，适应更潮湿的条件（每年降雨800至1500毫米），肥沃的土壤以及5摄氏度以上的温度。在受控温室条件下生长的植物的胚组织的Western blot分析清楚显示尽管30 kDa和32 kDa条带的数量不同，但存在两个脱水素带（分子量分别约为30、32、50和55 kDa），这对两个品种都是相同的。但是，当谷物分别来自其自然环境时，在萨哈马几乎看不到三个额外的条带（分子质量分别约为34、38和40 kDa），另一个较弱的条带（分子质量约为28 kDa）在Baer La Union中很明显。原位免疫定位显微镜在所有轴和子叶组织中检测到脱水素样蛋白。在亚细胞水平，在质膜，细胞质和细胞核中检测到脱水素。在细胞质中，发现脱水素与线粒体，粗面内质网池和质体膜有关。在蛋白质体的基质中还发现了脱水醇的存在。在细胞核中，脱水素与常染色质相关。通过检查属于高度对比环境的两个藜麦品种中脱水素的组成和亚细胞定位，我们得出的结论是，此处检测到的大多数脱水素是藜麦种子发育程序的组成成分，但其​​中一些（特别是34、38和40 kDa谱带）可能反映了与两个品种适应不同环境条件有关的定量分子差异。

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