...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Application of loop-mediated isothermal amplification (LAMP)-based technology for authentication of Catharanthus roseus (L.) G. Don
【24h】

Application of loop-mediated isothermal amplification (LAMP)-based technology for authentication of Catharanthus roseus (L.) G. Don

机译：基于环介导的等温扩增（LAMP）技术在长春花鉴定中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, loop-mediated isothermal amplification (LAMP)-based molecular marker was developed for authentication of Catharanthus roseus, a medicinal plant. Samples of this plant were collected from different geographical locations in India. Random amplified polymorphic deoxyribonucleic acid (DNA) analysis of collected samples was carried out with 25 random primers. A 610-bp DNA fragment, common to all accessions, was eluted, cloned, and sequenced. Four LAMP primers were designed on the basis of sequence of 610 bp DNA fragment. LAMP reaction, containing 10× Bst DNA polymerase reaction buffer, Bst DNA polymerase, four in-house designed primers, dNTPs, MgSO _4, and betaine, was incubated at 65°C for 1 h. The resulting amplicon was visualized by adding SYBR Green I to the reaction tube. The data showed confirmatory results. Since the assay method is simple, sensitive, and cost-effective, it is a feasible method for identifying and authentication of C. roseus.
机译：在这项研究中，开发了基于环介导的等温扩增（LAMP）的分子标记，用于鉴定药用植物长春花。该植物的样品是从印度的不同地理位置收集的。使用25个随机引物对收集的样品进行随机扩增的多态性脱氧核糖核酸（DNA）分析。洗脱，克隆并测序了所有种质共有的610 bp DNA片段。根据610 bp DNA片段的序列设计了四种LAMP引物。将含有10x Bst DNA聚合酶反应缓冲液，Bst DNA聚合酶，四种内部设计的引物，dNTPs，MgSO 4和甜菜碱的LAMP反应在65°C孵育1小时。通过向反应管中添加SYBR Green I，可以看到所得的扩增子。数据显示验证性结果。由于该测定方法简单，灵敏且具有成本效益，因此是鉴定和鉴定玫瑰假丝酵母的可行方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号