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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The importance of a validated standard methodology to define in vitro toxicity of nano-TiO _2 (Review)
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The importance of a validated standard methodology to define in vitro toxicity of nano-TiO _2 (Review)

机译：经验证的标准方法对定义纳米TiO _2体外毒性的重要性（综述）

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Several in vitro studies on the potential toxicity of nano-TiO _2 have been published and recent reviews have summarised them. Most of these reports concluded that physicochemical properties of nanoparticles are fundamental to their toxicological effects. No published review has compared in vitro tests with similar test strategies in terms of exposure duration and measured endpoints and for this reason we have attempted to assess the degree of homogeneity among in vitro tests and to assess if they afford reliable data to support risk assessment. The responses in different in vitro tests appeared to be unrelated to primary particle size. The biologically effective concentrations in different tests can be seen to differ by as many as two orders of magnitude and such differences could be explained either by different sensitivities of cell lines to nanoparticles or by effect of the test media. Our review indicates that even when the in vitro tests measure the same biomarkers with the same exposure duration and known primary particle sizes, it is insufficient merely to use such data for risk assessment. In the future, validated standard methods should include a limited number of cell lines and an obligatory selection of biomarkers. For routine purposes, it is important that assays can be easily conducted, false negatives and false positives are excluded and unbiased interpretation of results is provided. Papers published to date provide an understanding of the mode on nano-TiO _2 action but are not suitable for assessment and management of risk.
机译：已经发表了一些关于纳米TiO _2潜在毒性的体外研究，最近的综述对此进行了总结。这些报告大多数得出结论，纳米颗粒的理化性质对其毒理学作用至关重要。就暴露持续时间和测量的终点而言，没有发表的评论将体外测试与相似的测试策略进行比较，因此，我们试图评估体外测试之间的同质度，并评估它们是否提供可靠的数据来支持风险评估。在不同的体外测试中，这些反应似乎与初级粒径无关。可以看出在不同的测试中生物学有效浓度相差多达两个数量级，并且这种差异可以通过细胞系对纳米颗粒的不同敏感性或通过测试介质的作用来解释。我们的评论表明，即使在体外试验中以相同的暴露时间和已知的初级粒径测量相同的生物标志物，仅使用此类数据进行风险评估也是不够的。将来，经过验证的标准方法应包括数量有限的细胞系和必选的生物标志物。对于常规目的，重要的是，可以轻松进行测定，排除假阴性和假阳性，并提供无偏见的结果解释。迄今为止发表的论文提供了对纳米TiO _2作用方式的理解，但不适用于评估和管理风险。

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