...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >CYTOCHALASIN D BLOCKS CHROMOSOMAL ATTACHMENT TO THE SPINDLE IN THE GREEN ALGA OEDOGONIUM
【24h】

CYTOCHALASIN D BLOCKS CHROMOSOMAL ATTACHMENT TO THE SPINDLE IN THE GREEN ALGA OEDOGONIUM

机译：细胞松弛素D阻止染色体附着在绿藻紫铜的纺锤体上。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mitosis in living cells of Oedogonium observed by time-lapse, was blocked by cytochalasin D (CD; 25-100 mu g/ml). Normal prometaphase to anaphase takes 10-15 min; blockage of entry into anaphase by CD was reversible up to 2-2.5 h in CD and washout was followed within 10-20 min by normal anaphase and cytokinesis. After 3-6 h in CD, unseparated chromatids segregated randomly into two groups as the spindle slowly elongated considerably, becoming distorted and twisted. During this ''pseudoanaphase'', chromatids sometimes split irregularly and this was stimulated by late washout of CD. CD affected chromosomal attachment to the spindle. If applied al prophase and prometaphase, spindle fibres entered the nucleus; chromosomes moved vigorously and irregularly. A few achieved metaphase only briefly. Treatment at metaphase caused chromosomes to irregularly release and after random movement, all slowly gathered at either pole. Upon removal of CD, chromosomes rapidly achieved metaphase and anaphase A and B soon followed. If CD took effect during anaphase, chromatids detaching from the spindle oscillated rapidly along it; anaphase and cytokinesis (phycoplast formation) were delayed as the cell attempted to correct for abnormal chromosomal behaviour. Thus, CD prevents normal kinetochore attachment to the spindle and actin may be the target for this response. [References: 81]
机译：随时间推移观察到的Oedogonium活细胞中的有丝分裂被细胞松弛素D（CD; 25-100μg / ml）阻断。正常的前中期到后期需要10-15分钟; CD阻滞进入后期进入的过程在CD长达2-2.5小时内是可逆的，并且正常的后期和胞质分裂在10-20分钟内被清除。在CD中3-6小时后，未分离的染色单体随机分成两组，因为纺锤体缓慢缓慢伸长，变得扭曲扭曲。在这个“假后期”中，染色单体有时会不规则地分裂，这是由于CD的后期冲洗而引起的。 CD影响染色体与纺锤体的附着。如果在前期和前中期使用，纺锤体纤维进入核。染色体剧烈而不规则地移动。一些仅短暂地达到了中期。中期的处理导致染色体不规则地释放，并且在随机运动后，所有染色体都缓慢聚集在任一极。除去CD后，染色体迅速达到中期，后期很快达到A和B。如果CD在后期阶段生效，则从纺锤上脱离的染色单体会沿其快速振荡;如果CD在后期阶段生效，则染色质会从其上快速振荡。细胞试图纠正异常的染色体行为时，后期和胞质分裂（质体形成）被延迟。因此，CD阻止了正常的线粒体附着在纺锤体上，肌动蛋白可能是这种反应的靶点。 [参考：81]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号