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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >INHIBITION OF THIGMOSTIMULATED CELL DIFFERENTIATION WITH RGD-PEPTIDES IN UROMYCES GERMLINGS
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INHIBITION OF THIGMOSTIMULATED CELL DIFFERENTIATION WITH RGD-PEPTIDES IN UROMYCES GERMLINGS

机译：RGD-肽抑制尿嘧啶萌发过程中的高分化细胞分化

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摘要

Germlings of the plant pathogenic fungus Uromyces appendiculatus sense and respond to topographical signals of various substrata by undergoing a cell differentiation process that culminates in a structure termed an appressorium. In some cell systems, recognition and mediation of extracellular signals is via transmembrane glycoproteins known as integrins that often exhibit specific affinities to the tripeptide sequence Arg-Gly-Asp (RGD) found in several extracellular matrix components. Germlings grown on substrata inductive for appressorium formation in the presence of buffered synthetic peptides containing the amino acid sequence RGD, e.g., RGD, RGDS, GRGD, and GRGDGSPK (0.5-2.0 mM), were inhibited from developing appressoria. Two non-RGD peptides (GGGG and RGES) as well as two RGD peptides (GRGDS and RGDSPASSKP) did not inhibit appressorium formation. Germling growth was not significantly affected by any of the peptides. Furthermore, 0.5 mu m diameter micropipettes that are normally inductive for appressorium formation when positioned between the germling apex and the substratum did not induce appressorium formation when coated with the RGD peptide. Silanized micropipettes left uncoated or coated with RGES were inductive for appressorium formation. Those observations lead to the hypothesis that an integrin-like protein may be involved in the process of signaling for initiation of appressorium formation in Uromyces. An RGDSPC-affinity column was used to isolate proteins from Uromyces germlings with affinity to the RGD sequence. Elution with RGD or EDTA, but not with RGES, yielded at least 12 proteins of which one protein (95 kDa) expressed affinity on immunoblots to two different antibodies of beta(1)-integrin; one to the carboxyl-terminus of a synthetic peptide of integrin from chicken, and the other from the amino terminus of integrin from human placenta. [References: 72]
机译：植物病原性真菌Uromyces appendiculatus的萌发过程经历了细胞分化过程，并最终形成了称为Appressorium的结构，从而感知并响应各种地下的地形信号。在某些细胞系统中，细胞外信号的识别和介导是通过称为整合素的跨膜糖蛋白进行的，该蛋白通常表现出与在几种细胞外基质成分中发现的三肽序列Arg-Gly-Asp（RGD）的特异性亲和力。在含有氨基酸序列RGD，例如RGD，RGDS，GRGD和GRGDGSPK（0.5-2.0mM）的缓冲合成肽的存在下，在诱导诱导形成食欲的基质上生长的萌发被抑制了食欲的发展。两种非RGD肽（GGGG和RGES）以及两种RGD肽（GRGDS和RGDSPASSKP）均不抑制前庭的形成。萌发生长不受任何肽的影响。此外，当直径为0.5μm的微量移液管置于胚芽顶端和基底之间时，通常能诱导食堂形成，而当用RGD肽包被时，则不会诱导食堂形成。未涂覆或未涂覆RGES的硅烷化微量移液管感应诱导形成app。这些观察结果提出了这样的假说，即整联蛋白样蛋白可能参与了泌尿酵母中形成顶生菌的信号传导过程。 RGDSPC亲和柱用于分离与UGD序列具有亲和力的Uromyces幼苗中的蛋白质。用RGD或EDTA洗脱，但不使用RGES洗脱，至少产生12种蛋白质，其中一种蛋白质（95 kDa）在免疫印迹上表达对两种不同的beta（1）-integrin抗体的亲和力;一个来自鸡的整联蛋白合成肽的羧基末端，另一个来自人胎盘的整联蛋白的氨基末端。 [参考：72]

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